RAF1 Antikörper (N-Term)

Produktnummer ABIN452648

Der Maus Monoklonal anti-RAF1 Antikörper wird verwendet zum Nachweis von RAF1 in Proben von Human. Er wurde validiert für WB und EIA.

Kurzübersicht für RAF1 Antikörper (N-Term) (ABIN452648)

Target: RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser RAF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Klon: 163

Produktdetails anti-RAF1 Antikörper

Bindungsspezifität: N-Term

Spezifität: This antibody is specifically reactive to Raf-1 N-terminal (1-240 a.a.). Not suitable for the detection of Raf-1 expression and phosphorylation in Hela cells.

Produktmerkmale: Synonyms: C-RAF, Raf-1, cRaf, RAF proto-oncogene serine/threonine-protein kinase

Aufreinigung: Affinity chromatography on Protein G

Immunogen: Purified recombinant Raf-1 (mw: 75 kDa)

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: ELISA. Western Blot: 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with double distillated water to adjust the final concentration to 1,0 mg/mL.

Buffer: 0.01 M PBS, pH 7.2

Lagerung: -20 °C

Informationen zur Lagerung: Prior to and following reconstitution store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu RAF1

Target: RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Andere Bezeichnung: RAF1

Hintergrund: The Raf family of serine/threonine specific kinases is comprised of three members (aRaf, bRaf, and cRaf) that play a critical role in regulating cell growth and differentiation, and couple growth factor receptor stimulation to nuclear transcription factors via the Ras/mitogen activated protein kinase (MAPK) pathway. cRaf kinase (also known as Raf1) is a small GTPase like kinase of 73 kDa, and is a signal transducer of multiple extracellular stimuli that is regulated by several pathways, and that once activated, phosphorylates MEK which in turn phosphorylates ERK. Raf1 is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is part of the Ras dependent signaling pathway from receptors to the nucleus.Synonyms: C-RAF, RAF proto-oncogene serine/threonine-protein kinase, Raf-1, cRaf

Gen-ID: 5894

UniProt: P04049

Pathways: MAPK Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagie, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling