IKBKB Antikörper (Tyr199)

Produktnummer ABIN401637

Der Kaninchen Polyklonal Anti-IKBKB-Antikörper wurde für IF validiert. Er ist geeignet, IKBKB in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für IKBKB Antikörper (Tyr199) (ABIN401637)

Target: IKBKB (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IKBKB Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF)

Produktdetails anti-IKBKB Antikörper

Bindungsspezifität: Tyr199

Spezifität: IKK-β Antibody detects endogenous levels of total IKK-β protein.

Aufreinigung: Affinity chromatography

Immunogen: Synthesized non-phosphopeptide derived from human IKK-beta around the phosphorylation site of tyrosine 199 (Q-K-YP-T-V)

Anwendungsinformationen

Applikationshinweise: Immunofluorescence: 1/100 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu IKBKB

Target: IKBKB (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB))

Andere Bezeichnung: IKBKB / IKKB

Hintergrund: IKK beta (I-Kappa-B kinase-beta) is a member of the IKK complex which is composed of IKK alpha, IKK beta, IKK gamma and IKAP. Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquination. IKK beta has been shown to activate NF-kB and phosphorylate IKB alpha and beta. Phosphorylation of 2 sites at the activation loop of IKK beta is essential for activation of IKK by TNF and IL1. Once activated, IKK beta autophosphorylates which in turn decreases IKK activity and prevents prolonged activation of the inflammatory response. Additionally, IKK beta activity can also be regulated by MEKK1.Synonyms: I-kappa-B kinase 2, I-kappa-B-kinase beta, IKK-B, IKK-beta, IKK2, Inhibitor of nuclear factor kappa-B kinase subunit beta, NFKBIKB, Nuclear factor NF-kappa-B inhibitor kinase beta

Gen-ID: 3551

NCBI Accession: NP_001547

UniProt: O14920

Pathways: NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteine