HMGA1 Antikörper (Internal Region)

Produktnummer ABIN401529

Produktdetails anti-HMGA1 Antikörper

Target: HMGA1 (High Mobility Group AT-Hook 1 (HMGA1))

Bindungsspezifität: Internal Region

Reaktivität: Human, Maus, Ratte, Hund, Schwein, Rind (Kuh)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser HMGA1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequenz: c-LASKQEKDGT EK

Spezifität: This antibody detects HMGA1 (Internal).

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Dog, Pig, CowSpecies reactivity (tested):Human

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_665906.1, NP_665908.1, NP_002122.1, NP_665909.1, NP_665912.1, NP_665910.1. Genename: HMGA1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1: 32000. Western blot: Preliminary experiments gave an approx 50 kDa band in Human Testis andOvary lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot findan explanation in the literature for the band we observe given the calculated size of11.0 kDa according to isoform a (also called HMG-I, NP_665906.1, NP_665908.1,NP_665911.1) and 10.0 kDa according to isoform b( (also called HMG-Y, NP_002122.1,NP_665909.1, NP_665910.1, NP_665912.1). The 50 kDa band was successfully blocked byincubation with the immunizing peptide. We would appreciate any feedback from peoplein the field - have any results been reported with other antibodies/lysates? Have anyfurther splice variants/modified forms been reported?Immunohistochemistry: In paraffin embedded Human Placenta shows strong nuclearstaining. Recommended concentration, 4-6 μg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Details zu HMGA1

Target: HMGA1 (High Mobility Group AT-Hook 1 (HMGA1))

Andere Bezeichnung: HMGA1 / HMGIY (HMGA1 Produkte)

Synonyme: HMG-R antikoerper, HMGA1A antikoerper, HMGIY antikoerper, Hmgi antikoerper, Hmgiy antikoerper, hmga1 antikoerper, hmgiy antikoerper, wu:fb12b06 antikoerper, wu:fb18f11 antikoerper, wu:fc19f01 antikoerper, wu:fi38b05 antikoerper, zgc:85677 antikoerper, AL023995 antikoerper, Hmga1a antikoerper, Hmga1b antikoerper, Hmgy antikoerper, HMG-I(Y) antikoerper, HMG-Y antikoerper, HMGA1 antikoerper, zgc:153948 antikoerper, high mobility group AT-hook 1 antikoerper, high mobility group protein HMG-I/HMG-Y antikoerper, high mobility group AT-hook 1a antikoerper, high mobility group AT-hook 1b antikoerper, HMGA1 antikoerper, Hmga1 antikoerper, TSC_c24220 antikoerper, hmga1a antikoerper, LOC705105 antikoerper, hmga1b antikoerper

Hintergrund: HMGA1 (HMGI/Y) is a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. There are two splice isoforms of HMGA1 - the full length HMGA1a/HMGI and the shortened form HMGA1b/HMGY. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. HMGA1 is frequently acetylated and is found in the nucleus. Interacts with HIV-1 pre-integration complex.Synonyms: High mobility group AT-hook protein 1, High mobility group protein A1, High mobility group protein HMG-I/HMG-Y, High mobility group protein R

Gen-ID: 3159

NCBI Accession: NP_002122

UniProt: P17096

Pathways: Nuclear Hormone Receptor Binding, Autophagie