Species reactivity (expected):Mouse, Rat, Monkey, Chicken and Drosophila. Species reactivity (tested):Human.
Aufreinigung
Affinity Chromatography using acetyl-peptide coupled to agarose beads followed by solid phase adsorption against non-acetyl peptide.
Immunogen
This affinity purified HSP90 alpha antibody was prepared from Rabbit whole serum produced by repeated immunizations with a Synthetic peptide corresponding to amino acids surrounding K294 of Human Hsp90.
ELISA: 1/2,500-1/10,000. Western Blot: 1/500-1/2,500. Expect a band approx. 90 kDa in size corresponding to HSP90 rotein in the appropriate cell lysate or extract. Immunohistochemistry: 5-10 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
-20 °C
Target
HSP90AA1
(Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1))
EL52 antikoerper, HSP86 antikoerper, HSP89A antikoerper, HSP90A antikoerper, HSP90N antikoerper, HSPC1 antikoerper, HSPCA antikoerper, HSPCAL1 antikoerper, HSPCAL4 antikoerper, HSPN antikoerper, Hsp89 antikoerper, Hsp90 antikoerper, LAP2 antikoerper, Hsp86 antikoerper, Hspca antikoerper, htpG antikoerper, 86kDa antikoerper, 89kDa antikoerper, AL024080 antikoerper, AL024147 antikoerper, Hsp86-1 antikoerper, hsp4 antikoerper, HSP90 antikoerper, HSP90AA1 antikoerper, fb17b01 antikoerper, hsp90 antikoerper, hsp90a antikoerper, hsp90a.1 antikoerper, hsp90alpha antikoerper, wu:fb17b01 antikoerper, zgc:86652 antikoerper, Hsp90alpha antikoerper, heat shock protein 90 alpha family class A member 1 antikoerper, heat shock protein 90, alpha (cytosolic), class A member 1 antikoerper, Heat Shock Protein 90, cytosolic antikoerper, heat shock protein 90A antikoerper, molecular chaperone antikoerper, heat shock protein 90, alpha (cytosolic), class A member 1, tandem duplicate 1 antikoerper, heat shock protein HSP 90-alpha antikoerper, heat shock protein 90kDa alpha (cytosolic), class A member 1 antikoerper, HSP90AA1 antikoerper, Hsp90aa1 antikoerper, HSP90A antikoerper, hsp90A antikoerper, hsp90aa1.1 antikoerper, LOC108698781 antikoerper
Hintergrund
Hsp90 is a member of the heat shock protein 90 family, the members of which are highly conserved between isoforms and species. Hsp90 functions as a molecular chaperone and has ATPase activity. Hsp90 is a cytoplasmic protein that forms a homodimer in vivo and interacts with TOM34, AHSA1, HDAC6 and SMYD3. Several signal transduction pathways depend on Hsp90 function, including pathways involving erbB2, hypoxia sensitivity (Hif1 alpha), and steroid hormone receptors (for example, androgen, progesterone, glucocorticoid, and aryl-hydrocarbon). Recent reports show that Hsp90 from tumor cells has increased sensitivity to small molecule inhibitors (for example, 17AAG). The mechanism of the differential sensitivity of Hsp90 from normal versus tumor cells is unknown, although mutation has been ruled out. One possible mechanism may be differences in post-translational modification of tumor Hsp90. K294 was found to be acetylated in purified Hsp90 from SkBr3 cells, a breast cancer cell line.Synonyms: HSP86, HSP90A, HSPC1, HSPCA, Heat shock 86 kDa, Heat shock protein HSP 90-alpha, NY-REN-38