GST Antikörper

Produktnummer ABIN398843

Produktdetails anti-GST Antikörper

Target: GST (Glutathione S Transferase (GST))

Reaktivität: Schistosoma japonicum

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser GST Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Spezifität: Goat Anti-GST-tag Polyclonal Antibody detects GST fusion proteins in almost expression systems including bacterial expression system, mammalian expression system and other systems.

Aufreinigung: Immunoaffinity chromatography

Immunogen: Recombinant Glutathione S-Transferase (GST) from Schistosoma japonicum.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.2-1 µg/mLOther Applications: user-optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Referenzen für anti-GST Antikörper (ABIN398843)

Lichtenstein, Targan, Dubinsky, Rotter, Barken, Princen, Carroll, Brown, Stachelski, Chuang, Landers, Stempak, Singh, Silverberg: "Combination of genetic and quantitative serological immune markers are associated with complicated Crohn's disease behavior." in: Inflammatory bowel diseases, Vol. 17, Issue 12, pp. 2488-96, (2011) (PubMed).

Details zu GST

Target: GST (Glutathione S Transferase (GST))

Andere Bezeichnung: Glutathione-S-Transferase (GST) (GST Produkte)

Synonyme: GST antikoerper, GST 13-13 antikoerper, GST13 antikoerper, GST13-13 antikoerper, GSTK1-1 antikoerper, hGSTK1 antikoerper, 1500002K10Rik antikoerper, Gst antikoerper, Ptgds2 antikoerper, glutathione S-transferase kappa 1 antikoerper, microsomal glutathione S-transferase 1 antikoerper, hematopoietic prostaglandin D synthase antikoerper, GSTK1 antikoerper, Mgst1 antikoerper, Hpgds antikoerper

Hintergrund: Schistosoma japonicum glutathione S-transferase (GST) is a 26,000 Da enzyme that conjugates reduced glutathione to hydrophobic electrophiles. GST is an ideal fusion partner for production of foreign proteins in commercially available expression systems because it can be expressed in high levels in E. coli. The fusion proteins can be purified to homogeneity in a single step as the GST portion of the protein binds tightly to immobilized glutathione. Elution can be accomplished using free glutathione. The GST portion of the fusion protein can be separated from the protein of interest by using site-specific proteases. Antibodies directed against GST can be used in detecting the fusion protein during purification and to detect cleavage of GST from the protein of interest.Goat Anti-GST-tag Polyclonal Antibody is developed in goat using a recombinant Glutathione S-Transferase (GST) from Schistosoma japonicum.