GST Antikörper

Produktnummer ABIN3020561

Zitiert in 5+ Publikationen. Der Kaninchen Polyklonal anti-GST Antikörper (ABIN3020561) detektiert spezifisch GST in WB, IP und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Schistosoma japonicum.

Kurzübersicht für GST Antikörper (ABIN3020561)

Target: GST (Glutathione S Transferase (GST))

Reaktivität: Schistosoma japonicum

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GST Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Produktdetails anti-GST Antikörper

Aufreinigung: Affinity purification

Immunogen: Recombinant protein of human GST-Tag

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:1000-1:2000, IF: 1: 500, IP: 1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid freeze / thaw cycles

Lagerung: -20 °C / -80 °C

Referenzen für anti-GST Antikörper (ABIN3020561)

Yang, Gu, Cheung, Yan, Tong, Jiang, Yang: "InsP3R-SEC5 interaction on phagosomes modulates innate immunity to Candida albicans by promoting cytosolic Ca2+ elevation and TBK1 activity." in: BMC biology, Vol. 16, Issue 1, pp. 46, (2018) (PubMed).

Zhao, Tao, Qi, Xu, Yin, Peng: "Protective effect of dioscin against doxorubicin-induced cardiotoxicity via adjusting microRNA-140-5p-mediated myocardial oxidative stress." in: Redox biology, Vol. 16, pp. 189-198, (2018) (PubMed).

Gou, Kang, Dai, Wang, Li, Zhao, Zhang, Hua, Lu, Zhu, Li, Chen, Wu, Li, Fu, Li, Shi, Liu: "Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during Spermiogenesis." in: Cell, Vol. 169, Issue 6, pp. 1090-1104.e13, (2017) (PubMed).

Zhang, Cai, Lin, Tao, Jin: "Subcellular localization and interactions of Infectious Salmon Anemia Virus (ISAV) M1 and NEP as well as host Hsc70." in: Virology journal, Vol. 14, Issue 1, pp. 30, (2017) (PubMed).

Zhang, Wang, Zhong, Luo, Shang, Liu, Chen, Fang, Xiao: "Ubiquitin-specific Protease 15 Negatively Regulates Virus-induced Type I Interferon Signaling via Catalytically-dependent and -independent Mechanisms." in: Scientific reports, Vol. 5, pp. 11220, (2016) (PubMed).

Details zu GST

Target: GST (Glutathione S Transferase (GST))

Andere Bezeichnung: Glutathione-S-Transferase (GST)

Hintergrund: Plasmid vectors for the expression of coding regions of eukaryotic genes in E.coli are in common usage, such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. Forinstance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase(GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression bothin plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. Coli.