GST Antikörper (DyLight 680)

Produktnummer ABIN6720737

Hersteller Produkt- Nr.: 600-444-200

Produktdetails anti-GST Antikörper (DyLight 680)

Target: GST (Glutathione S Transferase (GST))

Reaktivität: Schistosoma japonicum

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GST Antikörper ist konjugiert mit DyLight 680

Applikation: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Hersteller Produkt- Nr.: 600-444-200

Hersteller: Rockland

Verwendungszweck: GST Antibody Dylight™ 680 Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.

Immunogen: The immunogen is full length GST isolated from Schistosoma japonicum.

Isotyp: IgG

Labeling Ratio: 2.9

Anwendungsinformationen

Applikationshinweise:

FLISA_Dilution: >1:10,000

IF_Microscopy_Dilution: >1:5,000

Other: User Optimized

Kommentare:

Anti-GST DyLight 680 Conjugated Antibody has been tested by western blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Referenzen für anti-GST Antikörper (DyLight 680) (ABIN6720737)

Waizenegger, Urulangodi, Lehmann, Reyes, Saugar, Tercero, Szakal, Branzei: "Mus81-Mms4 endonuclease is an Esc2-STUbL-Cullin8 mitotic substrate impacting on genome integrity." in: Nature communications, Vol. 11, Issue 1, pp. 5746, (2020) (PubMed).

Details zu GST

Target: GST (Glutathione S Transferase (GST))

Andere Bezeichnung: GST (GST Produkte)

Hintergrund:

Synonyms: rabbit anti-GST antibody DyLight™680 conjugation, DyLight™680 conjugated rabbit anti-GST antibody, Glutathione-S-Transferase

Background: GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors.  GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.