PAX8 Antikörper (Internal Region)

Produktnummer ABIN374803

Produktdetails anti-PAX8 Antikörper

Target: PAX8 (Paired Box 8 (PAX8))

Bindungsspezifität: Internal Region

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PAX8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Sequenz: C-AQPGSDKRKM DD

Spezifität: This antibody detects PAX8. It is expected to recognise all reported isoforms (NP_003457.1, NP_039245.1, NP_039246.1, NP_039247.1, NP_054698.1).

Kreuzreaktivität (Details): Species reactivity (tested):Human

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_003457.1, NP_039245.1, NP_039246.1, NP_039247.1, NP_054698.1 Genename: PAX8

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1: 32000. Western blot: 0.5-1.5 μg/mL. Approx 48 kDa + 48 kDa bands observed in lysates of cell lineHEK293 (calculated MW of 48.8 kDa according to PAX8A, NP_003457.1 and 41.8 kDaaccording to PAX8B, NP_039245.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Details zu PAX8

Target: PAX8 (Paired Box 8 (PAX8))

Andere Bezeichnung: PAX8 (PAX8 Produkte)

Synonyme: PAX8 antikoerper, XPax-8 antikoerper, XPax8 antikoerper, pax-8 antikoerper, PAX-8 antikoerper, Pax-8 antikoerper, paired box 8 antikoerper, paired box 8 L homeolog antikoerper, PAX8 antikoerper, pax8 antikoerper, Pax8 antikoerper, pax8.L antikoerper

Hintergrund: This protein is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain, an octapeptide, and a paired-type homeodomain. This family plays critical roles during fetal development and cancer growth. The specific function of the PAX8 is unknown but it may involve kidney cell differentiation, thyroid development, or thyroid dysgenesis. Alternative splicing in the gene by inclusion or exclusion of exons 7 and/or 8 has produced several known products but the biological significance of the variants is unknown. Several other splice variants have been proposed but the full nature of these products has not been described. Pax8 is also a marker of otic progenitor cells.Synonyms: Paired box protein Pax-8, Pax-8

Gen-ID: 7849

NCBI Accession: NP_003457

UniProt: Q06710

Pathways: Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Stem Cell Maintenance, Tube Formation