WNT3 Antikörper (Internal Region)

Produktnummer ABIN374685

Produktdetails anti-WNT3 Antikörper

Target: WNT3 (Wingless-Type MMTV Integration Site Family, Member 3 (WNT3))

Bindungsspezifität: Internal Region

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser WNT3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Spezifität: This antibody recognizes WNT3.

Kreuzreaktivität (Details): Species reactivity (expected):Rat, Canine (Dog).
Species reactivity (tested):Human, Mouse.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence CGRGHNTRTEKRKEK, from the internal region of the protein sequence according to NP_110380.1. Genename: WNT3

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/2000. Western Blot: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu WNT3

Target: WNT3 (Wingless-Type MMTV Integration Site Family, Member 3 (WNT3))

Andere Bezeichnung: WNT3 (WNT3 Produkte)

Synonyme: INT4 antikoerper, Ci-Wnt3 antikoerper, WNT3 antikoerper, int4 antikoerper, Xwnt3 antikoerper, wnt-3 antikoerper, wnt3l antikoerper, Xwnt-3 antikoerper, wnt3 antikoerper, Int-4 antikoerper, Wnt-3 antikoerper, wnt3-A antikoerper, Wnt family member 3 antikoerper, wingless-type MMTV integration site family, member 3 antikoerper, proto-oncogene Wnt-3 antikoerper, wingless-type MMTV integration site family member 3 antikoerper, Wnt family member 3A antikoerper, wingless-type MMTV integration site family member 3 L homeolog antikoerper, WNT3 antikoerper, Wnt3 antikoerper, wnt3 antikoerper, LOC468297 antikoerper, WNT3A antikoerper, wnt3.L antikoerper

Hintergrund: Wnts have diverse roles in governing cell fate, proliferation, migration, and polarity during development, particularly in embryogenesis and carcinogenesis. WNTs are transduced through at least three distinct intracellular signaling pathways, including the canonical Wnt/b-catenin, Wnt/Ca2+ and Wnt polarity pathways. Distinct sets of Wnt and Frizzled ligand-receptor pairs activate each of these pathways and lead to unique cellular responses. Wnt3 is expressed in embryonic mouse brain (with peak expression at around day 12 of gestation) and at low levels in the adult. It is present in the cerebellum during mouse development, and is restricted to the Purkinje cell layer in the adult mouse. Wnt3 is required for normal limb development and plays an important role in the establishment of the apical ectodermal ridge. In motorneurons, Wnt3 is a retrograde signal that controls terminal branching of muscle afferents. Wnt3 is highly expressed in human cancer cell lines including A549 (lung cancer), MKN45 (gastric cancer) and MCF7 (breast cancer). Wnt3 and Wnt3a are nearly 90 % identical at the amino acid level.Synonyms: INT-4, INT4, Proto-oncogene protein Wnt-3

Gen-ID: 7473, 9606

UniProt: P56703

Pathways: WNT Signalweg, Regulation of Cell Size