NUP50 Antikörper (C-Term)

Produktnummer ABIN374317

Produktdetails anti-NUP50 Antikörper

Target: NUP50 (Nucleoporin 50kDa (NUP50))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte, Rind (Kuh)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser NUP50 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequenz: C-ELHKILLEKK DA

Spezifität: This antibody detects NUP50 at C-term. It is expected to recognise both human isoforms of this protein.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Cow.
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_009103.2, NP_705931.1. Genename: NUP50

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.2-1 μg/mL. Approx 60 kDa band seen in Jurkat lysate (predicted MW 54 kDaaccording to NP_009103).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris buffered saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu NUP50

Target: NUP50 (Nucleoporin 50kDa (NUP50))

Andere Bezeichnung: NUP50 (NUP50 Produkte)

Synonyme: wu:fa56e03 antikoerper, wu:fb78a08 antikoerper, GB18194 antikoerper, npap60 antikoerper, npap60l antikoerper, Nup50 antikoerper, Rtp60 antikoerper, 1700030K07Rik antikoerper, AI413123 antikoerper, Npap60 antikoerper, NPAP60 antikoerper, NPAP60L antikoerper, nucleoporin 50 antikoerper, nuclear pore complex protein Nup50 antikoerper, nucleoporin 50kDa S homeolog antikoerper, nucleoporin 50kDa antikoerper, nup50 antikoerper, LOC410864 antikoerper, NUP50 antikoerper, nup50.S antikoerper, CpipJ_CPIJ012737 antikoerper, Nup50 antikoerper

Hintergrund: The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. NUP50 is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. NUP50 may serve as a binding site on the nuclear side of the pore complex for export receptor-cargo complexes. It interacts with multiple transport receptor proteins including p27Kip1. This interaction is required for correct intracellular transport and degradation of p27Kip1.Synonyms: NPAP60L, Nuclear pore-associated protein 60 kDa-like, Nucleoporin 50 kDa

Gen-ID: 10762

NCBI Accession: NP_009103

UniProt: Q9UKX7

Pathways: Tube Formation