FTH1 Antikörper (AA 221-232)

Produktnummer ABIN364417

Produktdetails anti-FTH1 Antikörper

Target: FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))

Bindungsspezifität: AA 221-232

Reaktivität: Human, Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser FTH1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human FTH1.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Opossum, Platypus (100%) Dolphin, Dog, Rabbit (92%) Panda, Cat, Armadillo (83%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-DKHTLGDSDNES from the C-terminus of human FTH1 (NP_002023.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Opossum, Platypus (100%), Dolphin, Dog, Rabbit (92%), Panda, Cat, Armadillo (83%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (4 μg/mL), Peptide-ELISA (1:8000), WB (0.03 - 0.1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 4 μg/mL. Peptide ELISA: antibody detection limit dilution 1:8000. Western Blot: Approx. 22kD band observed in Human Brain (Cerebellum), Liver and Placenta lysates (calculated MW of 21.2kD according to NP_002023.2). Recommended concentration: 0.03-0.1 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu FTH1

Target: FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))

Andere Bezeichnung: Ferritin / FTH1 (FTH1 Produkte)

Synonyme: FHC antikoerper, FTH antikoerper, FTHL6 antikoerper, PIG15 antikoerper, PLIF antikoerper, apoferritin antikoerper, ferritin antikoerper, fhc antikoerper, fth antikoerper, fth1 antikoerper, fthl6 antikoerper, ftn-2 antikoerper, pig15 antikoerper, plif antikoerper, fth1b antikoerper, Fth antikoerper, HFt antikoerper, MFH antikoerper, fb06g09 antikoerper, hm:zeh1145 antikoerper, wu:fb06g09 antikoerper, wu:fq18c10 antikoerper, zeh1145 antikoerper, ferritin heavy chain 1 antikoerper, ferritin, heavy polypeptide 1 L homeolog antikoerper, ferritin, heavy polypeptide 1 S homeolog antikoerper, ferritin heavy polypeptide 1 antikoerper, ferritin, heavy polypeptide 1a antikoerper, ferritin antikoerper, ferritin, heavy polypeptide 1 antikoerper, tudor domain containing 9 antikoerper, ferritin, heavy polypeptide 1 a antikoerper, FTH1 antikoerper, fth1.L antikoerper, fth1.S antikoerper, Fth1 antikoerper, fth1a antikoerper, EAMY_RS19690 antikoerper, fth1 antikoerper, TDRD9 antikoerper

Hintergrund: Name/Gene ID: FTH1

Synonyms: FTH1, Apoferritin, Ferritin H subunit, Ferritin heavy chain, Ferritin, heavy polypeptide 1, FHC, FTHL6, PIG15, PLIF, FTH

Gen-ID: 2495

NCBI Accession: NP_002023

UniProt: P02794

Pathways: Transition Metal Ion Homeostasis