FTH1 Antikörper (AA 58-180)

Produktnummer ABIN6939447

Dieser Maus Monoklonal Antikörper detektiert spezifisch FTH1 in WB und IHC. Es zeigt Reaktivität gegenüber Proben von Human und Ratte.

Kurzübersicht für FTH1 Antikörper (AA 58-180) (ABIN6939447)

Target: FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FTH1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: FTH-2081

Produktdetails anti-FTH1 Antikörper

Bindungsspezifität: AA 58-180

Verwendungszweck: Ferritin, Heavy Chain (FTH) (Microglia Marker) Antibody

Spezifität: Mammalian ferritins consist of 24 subunits made up of 2 types of poly-peptide chains, ferritin heavy chain and ferritin light chain, which each have unique functions. Ferritin heavy chains catalyze the first step in iron storage, the oxidationof FeI(II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe(III). The most prominent role of mammalian ferritins is to provide iron-buffering capacity to cells. In addition to iron buffering, heavy chain ferritin is also involved in the regulation of thymidine biosynthesis via increased expression of cytoplasmic serine hydroxymethyltransferase, which is a limiting factor in thymidylate synthesis in MCF-7 cells. Light chain ferritin is involved in cataracts by at least two mechanisms: hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of aging-related cataracts.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human FTH1 protein fragment (around aa 58-180) (exact sequence is proprietary)

Isotyp: IgG2c, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HepG2, HeLa, HL-60 or 293T cells. Pancreas, Liver, Cerebellum or Testis.

Known Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu FTH1

Target: FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))

Andere Bezeichnung: FTH1

Hintergrund:

Apoferritin, Cell proliferation-inducing gene 15 protein, Ferritin H subunit, Ferritin heavy chain, FHC, FRIH, FTH1, FTHL6, Iron overload autosomal dominant, PIG15, Placenta immunoregulatory factor, Proliferation inducing gene 15 protein (PLIF)

HeLa, HePG2, HL-60 or 293T cells. Pancreas, Liver, A-549 and A-431, Human Brain, Rat Brain, Human Kidney, Rat Kidney,Human Brain, Rat Brain, Human Kidney, Rat Kidney, Human Lungs, Rat Lungs and U87

Molekulargewicht: 21kDa

Gen-ID: 2495, 524910, 645560

UniProt: P02794

Pathways: Transition Metal Ion Homeostasis