RAF1 Antikörper (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (pSer259)

Details for Product anti-RAF1 Antibody No. ABIN361884, Anbieter: Anmelden zum Anzeigen
Antigen
  • CRAF
  • NS5
  • Raf-1
  • c-Raf
  • c-raf
  • craf
  • raf1
  • RAF1
  • Afr-1
  • Afr1
  • Raf
  • mKIAA0854
  • 6430402F14Rik
  • AA990557
  • BB129353
  • Craf1
  • D830050J10Rik
  • v-Raf
  • raf
  • cRaf
  • Raf-1 proto-oncogene, serine/threonine kinase
  • Raf-1 proto-oncogene, serine/threonine kinase a
  • zinc fingers and homeoboxes 2
  • v-raf-leukemia viral oncogene 1
  • Raf-1 proto-oncogene, serine/threonine kinase S homeolog
  • RAF1
  • Raf1
  • raf1a
  • raf1
  • Zhx2
  • raf1.S
Epitop
pSer259
82
74
48
48
45
39
34
34
34
31
31
22
21
20
19
18
16
15
15
15
14
14
10
10
9
8
8
7
6
5
5
5
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
887
456
427
27
25
18
18
16
9
8
8
5
4
4
2
2
1
1
1
Wirt
Kaninchen
835
62
11
6
2
Klonalität
Polyklonal
Konjugat
Dieser RAF1 Antikörper ist unkonjugiert
46
46
45
30
30
29
14
14
12
12
12
12
12
12
12
12
12
2
2
2
2
2
2
2
2
2
1
1
Applikation
Immunohistochemistry (IHC), Western Blotting (WB)
558
414
266
182
156
109
52
50
21
18
17
10
2
2
2
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Peptide sequence around phosphorylation site of pSer259 (S-T-S (p) -T-P) derived from Human Raf1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotyp IgG
Spezifität The antibody detects endogenous level of Raf-1 only when phosphorylated at serine 259.
Reinigung The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Plasmids, Primers & others Plasmide, Primers & weitere RAF1 products on genomics-online (e.g. as negative or positive controls)
Andere Bezeichnung Raf1 (RAF1 Antibody Abstract)
Hintergrund Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
Molekulargewicht 73 kDa
UniProt P04049
Pathways MAPK Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagie, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling
Applikations-hinweise Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Produkt verwendet in: Qiu, Wang, Han, Liu, Huang, Altaf, Qiu, Javed, Zheng, Chen, Li: "Prognostic impact of Raf-1 and p-Raf-1 expressions for poor survival rate in non-small cell lung cancer." in: Cancer science, Vol. 103, Issue 10, pp. 1774-9, 2012 (PubMed).

Allgemeine Veröffentlichungen Dougherty, Müller, Ritt, Zhou, Zhou, Copeland, Conrads, Veenstra, Lu, Morrison: "Regulation of Raf-1 by direct feedback phosphorylation." in: Molecular cell, Vol. 17, Issue 2, pp. 215-24, 2005 (PubMed).

Hekman, Fischer, Wennogle, Wang, Campbell, Rapp: "Novel C-Raf phosphorylation sites: serine 296 and 301 participate in Raf regulation." in: FEBS letters, Vol. 579, Issue 2, pp. 464-8, 2005 (PubMed).

Chong, Lee, Guan: "Positive and negative regulation of Raf kinase activity and function by phosphorylation." in: The EMBO journal, Vol. 20, Issue 14, pp. 3716-27, 2001 (PubMed).

King, Sun, Diaz, Barnard, Miao, Bagrodia, Marshall: "The protein kinase Pak3 positively regulates Raf-1 activity through phosphorylation of serine 338." in: Nature, Vol. 396, Issue 6707, pp. 180-3, 1998 (PubMed).

Mosbach: "Molecular imprinting." in: Trends in biochemical sciences, Vol. 19, Issue 1, pp. 9-14, 1994 (PubMed).

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