CAMK2G Antikörper (Calcium/calmodulin-Dependent Protein Kinase II gamma)

Details for Product anti-CAMK2G Antibody No. ABIN361644, Anbieter: Anmelden zum Anzeigen
Antigen
  • CAMK
  • CAMK-II
  • CAMKG
  • 5930429P18Rik
  • Camkg
  • calcium/calmodulin dependent protein kinase II gamma
  • calcium/calmodulin-dependent protein kinase II gamma
  • CAMK2G
  • Camk2g
Reaktivität
Rind (Kuh), Human, Maus, Ratte (Rattus)
144
113
110
38
3
2
2
2
1
1
1
1
1
Wirt
Maus
88
67
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser CAMK2G Antikörper ist unkonjugiert
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
Applikation
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
151
104
97
78
52
40
40
36
6
4
1
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Partially purified rat CaMKII
Klon 6G9
Isotyp IgG1
Spezifität Detects ~50-60 kDa. Recognizes both phosphorylated and non-phosphorylated forms.
Reinigung Protein G Purified
Plasmids, Primers & others Plasmide, Primers & weitere CAMK2G products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung CaMKII (CAMK2G Antibody Abstract)
Hintergrund CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).
Gen-ID 12322
NCBI Accession NP_033922
UniProt P11798
Pathways WNT Signalweg, Interferon-gamma Pathway, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of long-term Neuronal Synaptic Plasticity
Applikations-hinweise
  • WB (1:10000)
  • IHC (1:2000)
  • ICC/IF (1:50)
  • optimal dilutions for assays should be determined by the user.
Kommentare

0.1 μg/ml was sufficient for detection of CamKII in 20 μg rat brain tissue extract by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Bilder des Herstellers
 image for anti-Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G) antibody (ABIN361644) CamKII (6G9) in cultured cortical neurons.
Western Blotting (WB) image for anti-Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G) antibody (ABIN361644) western rat membrane 1 in 1000 CaMKII.
Produkt verwendet in: Feng, Szabo, Dziak, Opas: "Cytoskeletal disassembly and cell rounding promotes adipogenesis from ES cells." in: Stem cell reviews, Vol. 6, Issue 1, pp. 74-85, 2010 (PubMed).

Allgemeine Veröffentlichungen Hughes, Edin, Antonsson, Grundström: "Calmodulin-dependent kinase II mediates T cell receptor/CD3- and phorbol ester-induced activation of IkappaB kinase." in: The Journal of biological chemistry, Vol. 276, Issue 38, pp. 36008-13, 2001 (PubMed).

Means: "Regulatory cascades involving calmodulin-dependent protein kinases." in: Molecular endocrinology (Baltimore, Md.), Vol. 14, Issue 1, pp. 4-13, 2000 (PubMed).

Leonard, Lim, Hemsworth, Horne, Hell: "Calcium/calmodulin-dependent protein kinase II is associated with the N-methyl-D-aspartate receptor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 6, pp. 3239-44, 1999 (PubMed).

Makhinson, Chotiner, Watson, ODell: "Adenylyl cyclase activation modulates activity-dependent changes in synaptic strength and Ca2+/calmodulin-dependent kinase II autophosphorylation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 7, pp. 2500-10, 1999 (PubMed).

Shen, Meyer: "Dynamic control of CaMKII translocation and localization in hippocampal neurons by NMDA receptor stimulation." in: Science (New York, N.Y.), Vol. 284, Issue 5411, pp. 162-6, 1999 (PubMed).

Strack, Colbran: "Autophosphorylation-dependent targeting of calcium/ calmodulin-dependent protein kinase II by the NR2B subunit of the N-methyl- D-aspartate receptor." in: The Journal of biological chemistry, Vol. 273, Issue 33, pp. 20689-92, 1998 (PubMed).

Barria, Muller, Derkach, Griffith, Soderling: "Regulatory phosphorylation of AMPA-type glutamate receptors by CaM-KII during long-term potentiation." in: Science (New York, N.Y.), Vol. 276, Issue 5321, pp. 2042-5, 1997 (PubMed).

Tombes, Krystal: "Identification of novel human tumor cell-specific CaMK-II variants." in: Biochimica et biophysica acta, Vol. 1355, Issue 3, pp. 281-92, 1997 (PubMed).

Brocke, Srinivasan, Schulman: "Developmental and regional expression of multifunctional Ca2+/calmodulin-dependent protein kinase isoforms in rat brain." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 15, Issue 10, pp. 6797-808, 1995 (PubMed).

Edman, Schulman: "Identification and characterization of delta B-CaM kinase and delta C-CaM kinase from rat heart, two new multifunctional Ca2+/calmodulin-dependent protein kinase isoforms." in: Biochimica et biophysica acta, Vol. 1221, Issue 1, pp. 89-101, 1994 (PubMed).

Nghiem, Saati, Martens, Gardner, Schulman: "Cloning and analysis of two new isoforms of multifunctional Ca2+/calmodulin-dependent protein kinase. Expression in multiple human tissues." in: The Journal of biological chemistry, Vol. 268, Issue 8, pp. 5471-9, 1993 (PubMed).

Bennett, Kennedy: "Deduced primary structure of the beta subunit of brain type II Ca2+/calmodulin-dependent protein kinase determined by molecular cloning." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 7, pp. 1794-8, 1987 (PubMed).

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