PRKAR1B Antikörper (N-Term)

Produktnummer ABIN359120

Produktdetails anti-PRKAR1B Antikörper

Target: PRKAR1B (Protein Kinase, CAMP-Dependent, Regulatory, Type I, beta (PRKAR1B))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PRKAR1B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Spezifität: This antibody reacts to PKA R1 beta (PRKAR1B).

Aufreinigung: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human PRKAR1B.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu PRKAR1B

Target: PRKAR1B (Protein Kinase, CAMP-Dependent, Regulatory, Type I, beta (PRKAR1B))

Andere Bezeichnung: PRKAR1B (PRKAR1B Produkte)

Synonyme: PRKAR1B antikoerper, AI385716 antikoerper, RIbeta antikoerper, RGD1563094 antikoerper, PRKAR1 antikoerper, zgc:153624 antikoerper, prkar1 antikoerper, protein kinase cAMP-dependent type I regulatory subunit beta antikoerper, protein kinase, cAMP dependent regulatory, type I beta antikoerper, protein kinase cAMP-dependent type 1 regulatory subunit beta antikoerper, protein kinase, cAMP-dependent, regulatory, type I, beta antikoerper, protein kinase, cAMP-dependent, regulatory subunit type I beta S homeolog antikoerper, PRKAR1B antikoerper, prkar1b antikoerper, Prkar1b antikoerper, prkar1b.S antikoerper

Hintergrund: The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Cα, Cβ and Cγ, that each represent specific gene products. Cα and Cβ are closely related (93 % amino acid sequence similarity), whereas Cγ displays 83 % and 79 % similarity to Cα and Cβ, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex.Synonyms: PKA regulatory subunit I beta, PRKAR1, cAMP-dependent protein kinase type I-beta regulatory subunit

Gen-ID: 5575, 9606

UniProt: P31321

Pathways: Hedgehog Signalweg, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Myometrial Relaxation and Contraction, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma