RAF1 Antikörper (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (pSer494)

Details for Product anti-RAF1 Antibody No. ABIN358392, Anbieter: Anmelden zum Anzeigen
Antigen
  • CRAF
  • NS5
  • Raf-1
  • c-Raf
  • c-raf
  • craf
  • raf1
  • RAF1
  • Afr-1
  • Afr1
  • Raf
  • mKIAA0854
  • 6430402F14Rik
  • AA990557
  • BB129353
  • Craf1
  • D830050J10Rik
  • v-Raf
  • raf
  • cRaf
  • Raf-1 proto-oncogene, serine/threonine kinase
  • Raf-1 proto-oncogene, serine/threonine kinase a
  • zinc fingers and homeoboxes 2
  • v-raf-leukemia viral oncogene 1
  • Raf-1 proto-oncogene, serine/threonine kinase S homeolog
  • RAF1
  • Raf1
  • raf1a
  • raf1
  • Zhx2
  • raf1.S
Epitop
pSer494
82
74
48
48
44
39
34
34
33
30
30
22
21
21
20
18
16
15
15
15
14
14
10
10
9
8
8
7
5
5
5
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human
877
449
420
27
25
18
16
16
9
8
8
5
4
4
2
2
1
1
1
Wirt
Kaninchen
824
63
11
6
2
Klonalität
Polyklonal
Konjugat
Dieser RAF1 Antikörper ist unkonjugiert
46
46
45
30
30
29
14
14
12
12
12
12
12
12
12
12
12
2
2
2
2
2
2
2
2
2
1
1
Applikation
Dot Blot (DB), Enzyme Immunoassay (EIA)
548
404
268
181
156
102
51
50
21
17
17
11
2
2
2
2
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S494 of human RAF1.
Isotyp Ig Fraction
Spezifität This antibody detects RAF1 pSer494. Predicted to cross react with Mouse (100 % Antigen Homology).
Reinigung Affinity purification in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Andere Bezeichnung RAF1 (RAF1 Antibody Abstract)
Hintergrund Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.Synonyms: C-RAF, RAF proto-oncogene serine/threonine-protein kinase, Raf-1, cRaf
Molekulargewicht 73052 Da
Gen-ID 5894, 9606
UniProt P04049
Pathways MAPK Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagie, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling
Applikations-hinweise ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Bilder des Herstellers
 image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (pSer494) antibody (ABIN358392) anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (pSer494) antibody
Allgemeine Veröffentlichungen Rashdan, Fogel, McHenry, Sherman, Temkit, Lehman: "Improved stent characteristics for prophylaxis of post-ERCP pancreatitis." in: Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association, Vol. 2, Issue 4, pp. 322-9, 2004 (PubMed).

Dasgupta, Sun, Wang, Fusaro, Betts, Padmanabhan, Sebti, Chellappan: "Disruption of the Rb--Raf-1 interaction inhibits tumor growth and angiogenesis." in: Molecular and cellular biology, Vol. 24, Issue 21, pp. 9527-41, 2004 (PubMed).

Ku, Fu, Omary: "Raf-1 activation disrupts its binding to keratins during cell stress." in: The Journal of cell biology, Vol. 166, Issue 4, pp. 479-85, 2004 (PubMed).

Akula, Ford, Whitman, Hamden, Shelton, McCubrey: "Raf promotes human herpesvirus-8 (HHV-8/KSHV) infection." in: Oncogene, Vol. 23, Issue 30, pp. 5227-41, 2004 (PubMed).

Goetz, Harmon, ONeil, Burchill, Farrar: "STAT5 activation underlies IL7 receptor-dependent B cell development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 8, pp. 4770-8, 2004 (PubMed).

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