Erythrocyte Ankyrin Antikörper (AA 1971-1983)

Produktnummer ABIN343025

Produktdetails anti-Erythrocyte Ankyrin Antikörper

Target: Erythrocyte Ankyrin (ANK1) (Ankyrin 1, Erythrocytic (ANK1))

Bindungsspezifität: AA 1971-1983

Reaktivität: Human, Maus, Ratte, Hund, Kaninchen, Pferd, Rind (Kuh), Affe, Fledermaus, Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Erythrocyte Ankyrin Antikörper ist unkonjugiert

Applikation: ELISA

Spezifität: Human ANK1. This antibody is expected to recognise isoform 3 (NP_000028.3) and isoform 5 (NP_065211.2).

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Platypus (100%) Turkey (92%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-QIVKRASLKRGKQ from the C-terminus of human ANK1 (NP_000028.3, NP_065208.2, NP_065209.2, NP_065210.2, NP_065211.2, NP_065213.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Platypus (100%), Turkey (92%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: Peptide-ELISA (1:128000)

Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western Blot: Preliminary experiments gave approx. 19-23 kD bands in Human Skeletal Muscle lysates after 0.01 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for these bands we observe given the observed 28+30 kD bands in the literature for this tissue (PMID: 9430667) and given the calculated size of 17.6 kD according to NP_065211.2. The 19-22 kD bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

Not recommended for: IHC-P

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Erythrocyte Ankyrin

Target: Erythrocyte Ankyrin (ANK1) (Ankyrin 1, Erythrocytic (ANK1))

Andere Bezeichnung: ANK1 / Ankyrin (ANK1 Produkte)

Synonyme: CHANK1 antikoerper, wu:fa09h06 antikoerper, zgc:101835 antikoerper, ank antikoerper, sph1 antikoerper, sph2 antikoerper, ANK1 antikoerper, ANK antikoerper, SPH1 antikoerper, SPH2 antikoerper, Ank-1 antikoerper, nb antikoerper, pale antikoerper, ankyrin 1 antikoerper, ankyrin 1, erythrocytic a antikoerper, ankyrin 1 L homeolog antikoerper, ankyrin 1, erythrocytic antikoerper, ankyrin 1, erythroid antikoerper, ANK1 antikoerper, ank1a antikoerper, ank1 antikoerper, ank1.L antikoerper, Ank1 antikoerper

Hintergrund: Name/Gene ID: ANK1

Synonyms: ANK1, ANK-1, Ankyrin-1, Ankyrin-R, Erythrocyte ankyrin, ANK, Ankyrin, Ankyrin 1, erythrocytic, SPH2, SPH1

Gen-ID: 286

NCBI Accession: NP_000028, NP_065208, NP_065209, NP_065210, NP_065211, NP_065213

UniProt: P16157

Pathways: Synaptic Membrane