NR1H4 Antikörper (Internal Region)

Produktnummer ABIN334396

Produktdetails anti-NR1H4 Antikörper

Target: NR1H4 (Nuclear Receptor Subfamily 1, Group H, Member 4 (NR1H4))

Bindungsspezifität: Internal Region

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser NR1H4 Antikörper ist unkonjugiert

Applikation: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Verwendungszweck: Farnesoid X receptor / FXR

Sequenz: KSCREKTELT PDQQ

Kreuzreaktivität: Rind (Kuh), Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse: Verified

Immunogen: Peptide with sequence KSCREKTELTPDQQ, from the internal region of the protein sequence according to NP_005114.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Preliminary testing showed a band at approx 55 kDa in Human Kidney, Colon, Duodenum and Ileum lysate after 0.5 μg/mL antibody staining (calculated MW of 54.4 kDa according to NP_005114.1). Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of U2OS cells. Recommended concentration

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu NR1H4

Target: NR1H4 (Nuclear Receptor Subfamily 1, Group H, Member 4 (NR1H4))

Andere Bezeichnung: NR1H4 (NR1H4 Produkte)

Synonyme: zgc:110190 antikoerper, zgc:92742 antikoerper, NR1H4 antikoerper, BAR antikoerper, FXR antikoerper, HRR-1 antikoerper, HRR1 antikoerper, RIP14 antikoerper, AI957360 antikoerper, Fxr antikoerper, Rxrip14 antikoerper, nuclear receptor subfamily 1, group H, member 5 S homeolog antikoerper, nuclear receptor subfamily 1, group H, member 4 antikoerper, nuclear receptor subfamily 1 group H member 4 antikoerper, nr1h5.S antikoerper, nr1h4 antikoerper, NR1H4 antikoerper, Nr1h4 antikoerper

Hintergrund: Farnesoid X receptor, HRR1, NR1H4, nuclear receptor subfamily 1, group H, member 4 , BAR, FXR, HRR-1, RIP14 , farnesol receptor HRR-1

Gen-ID: 9971

NCBI Accession: NP_005114

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Carbohydrate Metabolic Process