RAF1 Antikörper (pSer642)

Produktnummer ABIN318805

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch RAF1 in WB. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Rind (Kuh), Huhn, Affe und Hund.

Kurzübersicht für RAF1 Antikörper (pSer642) (ABIN318805)

Target: RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Huhn, Affe, Hund

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RAF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-RAF1 Antikörper

Bindungsspezifität: pSer642

Spezifität: This antibody recognises Raf-1 phosphorylated at serine 642, a ubiquitously expressed 74 kDa member of the serine/threonine protein kinase family.

Aufreinigung: Purified

Immunogen: Phosphopeptide corresponding to animo acid sequence surrounding phosphorylated Ser 642 of rat Raf-1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western Blot: 1: 1000, detects a band of approximately 74 kDa.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 10 mM Hepes pH 7.5 containing 0.09 % Sodium Azide (NaN3), 0.01 % Bovine Serum Albumin, 50 % Glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu RAF1

Target: RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Andere Bezeichnung: RAF1

Hintergrund: Raf-1 is a MAP kinase kinase kinase and participates in the transduction of mitogenic signals from receptors on the cell membrane to the nucleus. Raf-1 is activated through phosphorylation by Ras and in turn phosphorylates MEK which activates ERK. ERK when activated regulates the expression of genes involved in cell cycle, differentiation, migration and apoptosis. As such Raf-1 is an important clinical target in cancer. Raf-1 activity can be downregulated by phosphorylation at serine residues 301 and 642.Synonyms: C-RAF, RAF proto-oncogene serine/threonine-protein kinase, Raf-1, cRaf

Gen-ID: 5894

NCBI Accession: NP_002871

UniProt: P04049

Pathways: MAPK Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagie, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling