Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Kreuzreaktivität
Human
Produktmerkmale
CYP3A7, CYP450 3A7 (EC 1.14.14.1), CYPIIIA7, P450-HFLA,CYP3A genes encode monooxygenases, enzymes which catalyze drug metabolism and the synthesis of cholesterol, steroids and other lipids. CYP3A (cytochrome P450, family 3, subfamily A), the most abundant P450 enzyme in human liver, is responsible for the metabolism of more than 50 % of all clinical drugs. CYP3A members localize in organs that associate with drug disposition, including the liver, gastrointestinal tract and kidney. The CYP3A cluster consists of four genes: CYP3A43, CYP3A4, CYP3A7 and CYP3A5, and two pseudogenes: CYP3A5P1 and CYP3A5P2. The CYP3A cluster maps to gene locus 7q21.3-q22.1.
Aufreinigung
Protein A/G Chromatography
Immunogen
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the C-terminus of the human CYP3A7 protein and mouse myeloma Ag8563 cells.
CYP3A7
Reaktivität: Human
WB
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Antibody can be used for Western blotting (1-2 μg/mL) and immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections (1-5 μg/mL). When used in conjunction with CYP3A5 (Cat. No. X2047M), can differentiate between the CYP3A family members, 3A5 and 3A7. Optimal concentration should be evaluated by serial dilutions.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
Provided as solution in phosphate buffered saline with 0.08 % sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C
Informationen zur Lagerung
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Target
CYP3A7
(Cytochrome P450, Family 3, Subfamily A, Polypeptide 7 (CYP3A7))