MUC1 Antikörper

Produktnummer ABIN306928

Produktdetails anti-MUC1 Antikörper

Target: MUC1 (Mucin 1 (MUC1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MUC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Kreuzreaktivität: Human

Produktmerkmale: Episialin,Muc1 is a heavily O-glycosylated transmembrane protein expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed in >90 % breast carcinomas and metastases. In normal mammary gland it is expressed in apical surface of glandular epithelium. In breast cancer Muc1 is overexpressed, is underglycosylated and the apical localization is lost. Muc1 is transcribed as a larger precursor which is cleaved to form a larger mucin like subunit (265-400 kDa) and a smaller subunit (14-28 kDa) noncovalently associated with each other. Transgenic Muc1 has been shown to associate with all four erbB receptors and localize with erbB1 (EGFR) in lactating glands1. Muc1 can act as a ligand for ICAM-1 on HUVEC cells, it can bind -catenin, GSK3 and it associates with Grb2-SOS upon phosphorylation.

Aufreinigung: Protein A/G Chromatography

Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a Muc1 60mer tandem repeat NH2-(VTSAPDTRPAPGSTAPPAHG)3-COOH and mouse myeloma SP2 cells.

Klon: VU-4H5

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Antibody can be used for immunohistochemistry on frozen and paraffin embedded tissues, ELISA and Western blotting. Optimal concentration should be evaluated by serial dilutions. Antibody can only detect Muc1 if the threonine residue of the PDTRPAP of the tandem repeat region is non-glycosylated4.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Details zu MUC1

Target: MUC1 (Mucin 1 (MUC1))

Andere Bezeichnung: Muc1 (MUC1 Produkte)

Synonyme: CA 15-3 antikoerper, CD227 antikoerper, EMA antikoerper, H23AG antikoerper, KL-6 antikoerper, MAM6 antikoerper, MCKD1 antikoerper, MUC-1 antikoerper, MUC-1/SEC antikoerper, MUC-1/X antikoerper, MUC1/ZD antikoerper, PEM antikoerper, PEMT antikoerper, PUM antikoerper, Muc-1 antikoerper, mucin 1, cell surface associated antikoerper, mucin 1, transmembrane antikoerper, MUC1 antikoerper, Muc1 antikoerper

UniProt: P15941

Pathways: Negative Regulation of intrinsic apoptotic Signaling