CYP11A1 Antikörper (C-Term)

Produktnummer ABIN3044268

Produktdetails anti-CYP11A1 Antikörper

Target: CYP11A1 (Cytochrome P450, Family 11, Subfamily A, Polypeptide 1 (CYP11A1))

Bindungsspezifität: AA 507-521, C-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CYP11A1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Verwendungszweck: Rabbit IgG polyclonal antibody for Cholesterol side-chain cleavage enzyme, mitochondrial(CYP11A1) detection. Tested with WB, IHC-P, IHC-F in Human.

Sequenz: ISFTFWPFNQ EATQQ

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Cholesterol side-chain cleavage enzyme, mitochondrial(CYP11A1) detection. Tested with WB, IHC-P, IHC-F in Human.
Gene Name: cytochrome P450, family 11, subfamily A, polypeptide 1
Protein Name: Cholesterol side-chain cleavage enzyme, mitochondrial

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CYP11A1(507-521aa ISFTFWPFNQEATQQ).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Referenzen für anti-CYP11A1 Antikörper (ABIN3044268)

He, Xu, Chen, Liu, Xi: "Abnormal apoptosis of trophoblastic cells is related to the up-regulation of CYP11A gene in placenta of preeclampsia patients." in: PLoS ONE, Vol. 8, Issue 3, pp. e59609, (2013) (PubMed).

Details zu CYP11A1

Target: CYP11A1 (Cytochrome P450, Family 11, Subfamily A, Polypeptide 1 (CYP11A1))

Andere Bezeichnung: CYP11A1 (CYP11A1 Produkte)

Synonyme: CYP11A antikoerper, CYP11A1 antikoerper, CYPXIA1 antikoerper, P450SCC antikoerper, Cyp11a antikoerper, Cypxia1 antikoerper, D9Ertd411e antikoerper, P450scc antikoerper, Scc antikoerper, cscc antikoerper, P450(scc) antikoerper, SSC antikoerper, cytochrome P450 family 11 subfamily A member 1 antikoerper, cholesterol side-chain cleavage enzyme, mitochondrial-like antikoerper, cytochrome P450 cholesterol side-chain cleavage antikoerper, cytochrome P450, family 11, subfamily a, polypeptide 1 antikoerper, cytochrome P450, family 11, subfamily A, polypeptide 1 antikoerper, CYP11A1 antikoerper, LOC101827747 antikoerper, Cyp11a1 antikoerper

Hintergrund: CYP11A1(Cytochrome p450, family 11, subfamily A, polypeptide 1), also called CYTOCHROME P450SCC, CYTOCHROME P450C11A1 or CYP11A, is a mitochondrial enzyme associated with the conversion of cholesterol to pregnenolone. CYP11A1 is a member of the cytochrome P450 superfamily of enzymes. The CYP11A1 gene is mapped on 15q24.1. Expression of the CYP11A1 gene may play a role in skin physiology and pathology and that cutaneous proopiomelanocortin activity may be autoregulated by a feedback mechanism involving glucocorticoids synthesized locally by Slominski et al. Using in vitro studies, CYP11A1 catalyzed the side-chain cleavage of 7-dehydrocholesterol to form 7-dehydropregnenolone. In addition, CYP11A1 catalyzed the metabolism of biologically inert vitamin D3, which is formed from 7-dehydrocholesterol, to form 2 hydroxylated products, 20-hydroxyvitamin D3 and 20, 22-dihydroxyvitamin D3. Mutations in the CYP11A1 gene cause congenital adrenal insufficiency with partial or complete 46, XY sex reversal.

Synonyms: Cholesterol 20 22 desmolase antibody|Cholesterol desmolase antibody|Cholesterol monooxygenase(side chain cleaving) antibody|Cholesterol side chain cleavage enzyme antibody|Cholesterol side chain cleavage enzyme mitochondrial antibody|Cholesterol side-chain cleavage enzyme antibody|CP11A_HUMAN antibody|CYP 11A antibody|CYP11A antibody|CYP11A1 antibody|CYPXIA1 antibody|Cytochrome P450 11A1 antibody|Cytochrome P450 11A1 mitochondrial antibody|Cytochrome P450 family 11 subfamily A polypeptide 1 antibody|Cytochrome P450 subfamily XIA(cholesterol side chain cleavage) antibody|Cytochrome P450 subfamily XIA antibody|Cytochrome P450(scc) antibody|Cytochrome P450C11A1 antibody|mitochondrial antibody|P450(scc) antibody|P450SCC antibody|Steroid 20 22 lyase antibody

UniProt: P05108

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process, Cellular Response to Molecule of Bacterial Origin