HSPH1 Antikörper (C-Term)

Produktnummer ABIN3044218

Produktdetails anti-HSPH1 Antikörper

Target: HSPH1 (Heat Shock 105kDa/110kDa Protein 1 (HSPH1))

Bindungsspezifität: AA 713-733, C-Term

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HSPH1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Verwendungszweck: Rabbit IgG polyclonal antibody for Heat shock protein 105 kDa(HSPH1) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.

Sequenz: EVMEWMNNVM NAQAKKSLDQ D

Kreuzreaktivität (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Produktmerkmale: Rabbit IgG polyclonal antibody for Heat shock protein 105 kDa(HSPH1) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.
Gene Name: heat shock 105 kDa/110 kDa protein 1
Protein Name: Heat shock protein 105 kDa

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Hsp105(713-733aa EVMEWMNNVMNAQAKKSLDQD), different from the related mouse sequence by one amino acid, and different from the related rat sequence by two amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu HSPH1

Target: HSPH1 (Heat Shock 105kDa/110kDa Protein 1 (HSPH1))

Andere Bezeichnung: HSPH1 (HSPH1 Produkte)

Synonyme: hsp105 antikoerper, hsp105a antikoerper, hsp105b antikoerper, hsp110 antikoerper, hsph1b antikoerper, Hsp105 antikoerper, HSP105 antikoerper, HSP105A antikoerper, HSP105B antikoerper, NY-CO-25 antikoerper, 105kDa antikoerper, AI790491 antikoerper, Hsp110 antikoerper, hsp-E7I antikoerper, hsph1 antikoerper, hsph1a antikoerper, heat shock protein family H (Hsp110) member 1 antikoerper, heat shock protein family H (Hsp110) member 1 S homeolog antikoerper, heat shock protein 105 kDa antikoerper, heat shock 105kDa/110kDa protein 1 antikoerper, heat shock protein family H (Hsp110) member 1 L homeolog antikoerper, hsph1 antikoerper, hsph1.S antikoerper, HSPH1 antikoerper, LOC100546590 antikoerper, Hsph1 antikoerper, hsph1.L antikoerper

Hintergrund: HSP105(HEAT-SHOCK 105/110-KD PROTEIN 1), also called HSPH1 or HSP110, is a protein that in humans is encoded by the HSPH1 gene. Immunohistochemical analysis localizes HSP105 mainly in the cytoplasm. Database analysis indicates that both HSP105 isoforms are highly conserved during evolution. By analysis of radiation hybrids and human/rodent hybrid cell lines, the HSPH1 gene is mapped to chromosome 13. Both HSP105-alpha and HSP105-beta are upregulated in HeLa cells exposed to heat shock. HSP105-alpha, but not HSP105-beta, is also upregulate in response to other cell stresses. Following heat shock, HSP105 relocalizes from a cytoplasmic to perinuclear position. Besides, HSP110 may thus constitute a major determinant for both prognosis and treatment response in colorectal cancer.

Synonyms: Antigen NY CO 25 antibody|Antigen NY-CO-25 antibody|DKFZp686M05240 antibody|Heat shock 105kD alpha antibody|Heat shock 105kD antibody|Heat shock 105kD beta antibody|Heat shock 105 kDa protein 1 antibody|Heat shock 105 kDa protein antibody|Heat shock 105 kDa/110 kDa protein 1 antibody|Heat shock 110 kDa protein antibody|Heat shock 110 kDa protein antibody|Heat shock protein 105 kDa antibody|HS105_HUMAN antibody|HSP105 antibody|HSP105A antibody|HSP105B antibody|HSP110 antibody|HSPH 1 antibody|HSPH1 antibody|KIAA0201 antibody|NY CO 25 antibody

UniProt: Q92598