COL1A1 Antikörper (C-Term)

Produktnummer ABIN3043986

Produktdetails anti-COL1A1 Antikörper

Target: COL1A1 (Collagen, Type I, alpha 1 (COL1A1))

Bindungsspezifität: AA 1203-1218, C-Term

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser COL1A1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Verwendungszweck: Rabbit IgG polyclonal antibody for Collagen alpha-1(I) chain(COL1A1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.

Sequenz: QPPQEKAHDG GRYYRA

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Collagen alpha-1(I) chain(COL1A1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: collagen, type I, alpha 1
Protein Name: Collagen alpha-1(I) chain

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Collagen I(1203-1218aa QPPQEKAHDGGRYYRA), different from the related rat and mouse sequences by two amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species:Human, Mouse, The detection limit for COL1A1 is approximately 0.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Predicted Species: Human, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Referenzen für anti-COL1A1 Antikörper (ABIN3043986)

Huang, Zhang, Liu, Hao, Jiang, Han: "Granulocyte-Colony Stimulating Factor (G-CSF) Accelerates Wound Healing in Hemorrhagic Shock Rats by Enhancing Angiogenesis and Attenuating Apoptosis." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 23, pp. 2644-2653, (2018) (PubMed).

Yi, Liu, Tang, Li, Qin, Yuan: "Irradiated Human Umbilical Vein Endothelial Cells Undergo Endothelial-Mesenchymal Transition via the Snail/miR-199a-5p Axis to Promote the Differentiation of Fibroblasts into Myofibroblasts." in: BioMed research international, Vol. 2018, pp. 4135806, (2018) (PubMed).

Wang, Fan, Li, Wang, Gao, Yang: "Oxidative stress promotes myocardial fibrosis by upregulating KCa3.1 channel expression in AGT-REN double transgenic hypertensive mice." in: Pflügers Archiv : European journal of physiology, (2017) (PubMed).

Tan, Lu, Miao, Lei, Zheng, Zhao: "Effect of NGF From Venom of Chinese Cobra (Naja Atra)on Chondrocytes Proliferation and Metabolism In Vitro." in: Journal of cellular biochemistry, (2017) (PubMed).

Zeng, Xiao, Deng, Li: "Chondroprotective Effects and Multitarget Mechanisms of Fu Yuan Capsule in a Rat Osteoarthritis Model." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2017, pp. 8985623, (2017) (PubMed).

Huang, Liu, Hao, Chen, Zhizhong, Li, Song, Xiang, Jiang, Fu, Han: "Preferred M2 Polarization by ASC-Based Hydrogel Accelerated Angiogenesis and Myogenesis in Volumetric Muscle Loss Rats." in: Stem cells international, Vol. 2017, pp. 2896874, (2017) (PubMed).

Cui, Pan, Pan: "Danggui Buxue Extract-Loaded Liposomes in Thermosensitive Gel Enhance In Vivo Dermal Wound Healing via Activation of the VEGF/PI3K/Akt and TGF-β/Smads Signaling Pathway." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2017, pp. 8407249, (2017) (PubMed).

Shang, Liu, Liu, Zhang, Mu, Zhu, Liang, Zhai, Ding, Li, Wen: "Acetyl-11-Keto-β-Boswellic Acid Attenuates Prooxidant and Profibrotic Mechanisms Involving Transforming Growth Factor-β1, and Improves Vascular Remodeling in Spontaneously Hypertensive Rats." in: Scientific reports, Vol. 6, pp. 39809, (2016) (PubMed).

Zhang, Teng, Liu, Zhang, Liu: "Gene expression profile analyze the molecular mechanism of CXCR7 regulating papillary thyroid carcinoma growth and metastasis." in: Journal of experimental & clinical cancer research : CR, Vol. 34, pp. 16, (2015) (PubMed).

Liu, Lu, Wu, Zheng: "Chondroprotective effects of taurine in primary cultures of human articular chondrocytes." in: The Tohoku journal of experimental medicine, Vol. 235, Issue 3, pp. 201-13, (2015) (PubMed).

Zhou, Zhang, Xu, Wang: "Curcumin ameliorates renal fibrosis by inhibiting local fibroblast proliferation and extracellular matrix deposition." in: Journal of pharmacological sciences, Vol. 126, Issue 4, pp. 344-50, (2015) (PubMed).

Yang, Ni, Duan, Wang, Chen, Chen, Chaugai, Zeldin, Tang, Wang: "CYP epoxygenase 2J2 prevents cardiac fibrosis by suppression of transmission of pro-inflammation from cardiomyocytes to macrophages." in: Prostaglandins & other lipid mediators, Vol. 116-117, pp. 64-75, (2015) (PubMed).

Zhang, Li, Wu: "The in vitro and in vivo cementogenesis of CaMgSi?O? bioceramic scaffolds." in: Journal of biomedical materials research. Part A, Vol. 102, Issue 1, pp. 105-16, (2015) (PubMed).

Li, Yang, Qian, Wang, Han, Hao, Shu, Zhang, Yao, Wang: "Iota-carrageenan/chitosan/gelatin scaffold for the osteogenic differentiation of adipose-derived MSCs in vitro." in: Journal of biomedical materials research. Part B, Applied biomaterials, Vol. 103, Issue 7, pp. 1498-510, (2015) (PubMed).

Ye, Wang, Zhang, Song, Yang, Li, Li, Su, Yang, Ma: "Over-expression of calpastatin inhibits calpain activation and attenuates post-infarction myocardial remodeling." in: PLoS ONE, Vol. 10, Issue 3, pp. e0120178, (2015) (PubMed).

Jiang, Chen, Li, An, Jiao: "Ulinastatin attenuates renal interstitial inflammation and inhibits fibrosis progression in rats under unilateral ureteral obstruction." in: Molecular medicine reports, Vol. 10, Issue 3, pp. 1501-8, (2014) (PubMed).

Lv, Wu, Zhou, Shao, Wang, Wang: "Alpha Lipoic Acid Modulated High Glucose-Induced Rat Mesangial Cell Dysfunction via mTOR/p70S6K/4E-BP1 Pathway." in: International journal of endocrinology, Vol. 2014, pp. 658589, (2014) (PubMed).

Liu, Liu, Lin, Wei, Zheng: "Effect of JEZTC, a synthetic compound, on proliferation and phenotype maintenance of rabbit articular chondrocytes in vitro." in: In vitro cellular & developmental biology. Animal, Vol. 50, Issue 10, pp. 982-91, (2014) (PubMed).

Han, Wang, Wang, Li, Zang: "Association between pelvic organ prolapse and stress urinary incontinence with collagen." in: Experimental and therapeutic medicine, Vol. 7, Issue 5, pp. 1337-1341, (2014) (PubMed).

Ge, Wang, Wu, Fan, Chen, Zhu: "?-catenin is overexpressed in hepatic fibrosis and blockage of Wnt/?-catenin signaling inhibits hepatic stellate cell activation." in: Molecular medicine reports, Vol. 9, Issue 6, pp. 2145-51, (2014) (PubMed).

Details zu COL1A1

Target: COL1A1 (Collagen, Type I, alpha 1 (COL1A1))

Andere Bezeichnung: COL1A1 (COL1A1 Produkte)

Synonyme: OI4 antikoerper, Col1a-1 antikoerper, Cola-1 antikoerper, Cola1 antikoerper, Mov-13 antikoerper, Mov13 antikoerper, COLIA1 antikoerper, col1a1 antikoerper, MGC52532 antikoerper, COL1A1 antikoerper, COL1A2 antikoerper, alpha1(I) antikoerper, cb21 antikoerper, sb:cb21 antikoerper, sb:cb384 antikoerper, wu:fa99c12 antikoerper, wu:fb02c06 antikoerper, alpha3(I) antikoerper, col1a3 antikoerper, hm:zeh0348 antikoerper, wu:fa95h05 antikoerper, wu:fd02a10 antikoerper, wu:fj59a10 antikoerper, collagen type I alpha 1 chain antikoerper, collagen, type I, alpha 1 antikoerper, collagen, type I, alpha 1 S homeolog antikoerper, collagen alpha-1(I) chain antikoerper, collagen type I alpha 2 chain antikoerper, collagen, type I, alpha 1a antikoerper, collagen 1a1 antikoerper, collagen, type I, alpha 1b antikoerper, COL1A1 antikoerper, Col1a1 antikoerper, col1a1.S antikoerper, LOC397571 antikoerper, COL1A2 antikoerper, col1a1a antikoerper, col1a1 antikoerper, col1a1b antikoerper

Hintergrund: Collagen, type I, alpha 1, also known as COL1A1, is a human gene that encodes the major component of type I collagen, the fibrillar collagen found in most connective tissues, including cartilage. This gene is mapped to 17q21.33. This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis.

Synonyms: Alpha 1 type I collagen antibody|Alpha 2 type I collagen antibody|Alpha-1 type I collagen antibody|CO1A1_HUMAN antibody|COL1A1 antibody|COL1A2 antibody|Collagen alpha-1(I) chain antibody|Collagen I alpha 1 polypeptide antibody|Collagen I alpha 2 polypeptide antibody|Collagen Of Skin Tendon And Bone antibody|Collagen Type 1 antibody|Collagen type I alpha 1 antibody|Collagen type I alpha 2 antibody|OI4 antibody|Osteogenesis Imperfecta Type IV antibody|Pro alpha 1(I) collagen antibody|Type I procollagen antibody

UniProt: P02452

Pathways: Sensory Perception of Sound, Autophagie, Growth Factor Binding