IKK alpha Antikörper (AA 411-745)
Kurzübersicht für IKK alpha Antikörper (AA 411-745) (ABIN3043812)
Target
Alle IKK alpha (CHUK) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Bindungsspezifität
- AA 411-745
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Verwendungszweck
- Anti-IKK alpha/CHUK Antibody Picoband®
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Kreuzreaktivität (Details)
- No cross-reactivity with other proteins
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Produktmerkmale
- Anti-IKK alpha/CHUK Antibody Picoband® (ABIN3043812). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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Aufreinigung
- Immunogen affinity purified.
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Immunogen
- E.coli-derived human IKK alpha recombinant protein (Position: V411-E745). Human IKK alpha shares 98% amino acid (aa) sequence identity with mouse IKK alpha.
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Isotyp
- IgG
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Applikationshinweise
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Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human
1. Mock BA, Connelly MA, McBride OW, Kozak CA, Marcu KB (May 1995). "CHUK, a conserved helix-loop-helix ubiquitous kinase, maps to human chromosome 10 and mouse chromosome 19". Genomics27 (2): 348-51. 2. Häcker H, Karin M (October 2006). "Regulation and function of IKK and IKK-related kinases".Sci. STKE 2006 (357): re13. 3. Descargues P, Sil AK, Karin M (October 2008). "IKKα, a critical regulator of epidermal differentiation and a suppressor of skin cancer". EMBO J. 27 (20): 2639-47. 4. Zhu F, Park E, Liu B, Xia X, Fischer SM, Hu Y (February 2009). "Critical role of IkappaB kinase alpha in embryonic skin development and skin carcinogenesis". Histol. Histopathol. 24 (2): 265-71. -
Kommentare
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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Konzentration
- 500 μg/mL
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Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
- Avoid repeated freezing and thawing.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))
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Andere Bezeichnung
- CHUK
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Hintergrund
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Synonyms: Inhibitor of nuclear factor kappa-B kinase subunit alpha,I-kappa-B kinase alpha,IKK-A,IKK-alpha,IkBKA,IkappaB kinase,2.7.11.10,Conserved helix-loop-helix ubiquitous kinase,I-kappa-B kinase 1,IKK1,Nuclear factor NF-kappa-B inhibitor kinase alpha,NFKBIKA,Transcription factor 16,TCF-16,CHUK,IKKA, TCF16,
Tissue Specificity: Widely expressed.
Background: IKKA, also known as conserved helix-loop-helix ubiquitous kinase (CHUK) or IKBKA, is a protein kinase that in humans is encoded by the CHUK gene. It is mapped to 10q24.31. This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. IKKA is part of the IκB kinase complex that plays an important role in regulating the NF-κB transcription factor. However, IKKA also has many additional cellular targets, and is thought to function independently of the NF-κB pathway to regulate epidermal differentiation.
Sequence Similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
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Molekulargewicht
- 85 kDa
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Gen-ID
- 1147
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UniProt
- O15111
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Pathways
- PI3K-Akt Signalweg, NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteine
Target
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