DIO2 Antikörper (AA 217-228)

Produktnummer ABIN303850

Produktdetails anti-DIO2 Antikörper

Target: DIO2 (Deiodinase, Iodothyronine, Type II (DIO2))

Bindungsspezifität: AA 217-228

Reaktivität: Human, Maus, Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser DIO2 Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraformaldehyde) (IHC (pfa))

Spezifität: Human DIO2. This antibody is expected to recognise both reported isoform a (NP_000784.2 and NP_054644.1) and isoform b (NP_001007024.1).

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Pig (100%) Mouse, Rat, Sheep, Goat, Panda, Bovine, Rabbit, Chicken, Frog (92%) Hamster, Xenopus (83%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide EVKKHQNQEDRC from an internal region of human DIO2 (NP_000784.2, NP_054644.1, NP_001007024.1). Percent identity by BLAST analysis: Human, Gorilla, Pig (100%), Mouse, Rat, Sheep, Goat, Panda, Bovine, Rabbit, Chicken, Frog (92%), Hamster, Xenopus (83%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-Pfa, Peptide-ELISA (1:128000)

Usage: IHC: Cryo-sectioned (4 % PFA-fixed) Mouse Brain (Hippocampus) shows staining in the granular layer of the dentate gyrus. Recommended concentration, 2-3 μg/mL. Data obtained from anonymous customer. Western blot: Preliminary experiments gave an approx 65-70 kDa band in Human Heart and Thyroid lysates after 0.05 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 30.5 kDa according to NP_NP_000784.2 and of 34.6 kDa according to NP_001007024.1. The 65-70 kDa band was successfully blocked by incubation with the immunizing peptide.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu DIO2

Target: DIO2 (Deiodinase, Iodothyronine, Type II (DIO2))

Andere Bezeichnung: DIO2 (DIO2 Produkte)

Synonyme: 5DII antikoerper, D2 antikoerper, DIOII antikoerper, SelY antikoerper, TXDI2 antikoerper, AI324267 antikoerper, Deio2 antikoerper, cb684 antikoerper, DIO2 antikoerper, dio2-A antikoerper, 5dii antikoerper, sely antikoerper, txdi2 antikoerper, iodothyronine deiodinase 2 antikoerper, deiodinase, iodothyronine, type II antikoerper, deiodinase, iodothyronine, type 2 L homeolog antikoerper, deiodinase, iodothyronine, type 2 antikoerper, type II iodothyronine deiodinase antikoerper, DIO2 antikoerper, Dio2 antikoerper, dio2 antikoerper, dio2.L antikoerper, LOC100861220 antikoerper

Hintergrund: Name/Gene ID: DIO2

Synonyms: DIO2, 5DII, D2, DIOII, ITDI2, Type 2 DI, TXDI2, Type-II 5-deiodinase, SelY, Type-II 5deiodinase, Thyroxine deiodinase, type II

Gen-ID: 1734

NCBI Accession: NP_000784, NP_054644, NP_001007024

UniProt: Q92813

Pathways: Hormone Transport, Hormone Activity