PGK1 Antikörper (Middle Region)

Produktnummer ABIN3032222

Produktdetails anti-PGK1 Antikörper

Target: PGK1 (Phosphoglycerate Kinase 1 (PGK1))

Bindungsspezifität: Middle Region

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PGK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Aufreinigung: Antigen affinity

Immunogen: An amino acid sequence from the middle region of human Phosphoglycerate Kinase 1 (FGTAHRAHSSMVGVN) was used as the immunogen for this PGK1 antibody (100% homologous in human, mouse and rat).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting amounts. Titration of the PGK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL, IHC-P: 0.5-1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: -20 °C

Informationen zur Lagerung: After reconstitution, the PGK1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Details zu PGK1

Target: PGK1 (Phosphoglycerate Kinase 1 (PGK1))

Andere Bezeichnung: PGK1 (PGK1 Produkte)

Synonyme: MIG10 antikoerper, PGKA antikoerper, Pgk-1 antikoerper, Pgk antikoerper, PGK antikoerper, wu:fd59b07 antikoerper, wu:fj36g06 antikoerper, zgc:56252 antikoerper, zgc:77899 antikoerper, PGK1 antikoerper, phosphoglycerate kinase 1 antikoerper, pgk2 antikoerper, pgka antikoerper, phosphoglycerate kinase 1 antikoerper, phosphoglycerate kinase 1 L homeolog antikoerper, PGK1 antikoerper, Pgk1 antikoerper, pgk1 antikoerper, LOC100348124 antikoerper, pgk1.L antikoerper

Hintergrund: Phosphoglycerate Kinase 1, also known as PGKA, is an enzyme that in humans is encoded by the PGK1 gene. Willard et al.(1985) used a cDNA for human PGK to map the functional PGK1 gene to Xq13. Disulfide bonds in secreted proteins are considered to be inert because of the oxidizing nature of the extracellular milieu. An exception to this rule is a reductase secreted by tumor cells that reduces disulfide bonds in the serine proteinase plasmin. Lay et al.(2000) showed that the plasmin reductase isolated from conditioned medium of fibrosarcoma cells is the glycolytic enzyme phosphoglycerate kinase. Recombinant phosphoglycerate kinase had the same specific activity as the fibrosarcoma-derived protein. Plasma of mice bearing fibrosarcoma tumors contained several-fold more phosphoglycerate kinase, as compared with mice without tumors.

UniProt: P00558

Pathways: Cellular Glucan Metabolic Process