NOL3 Antikörper (AA 91-106)

Produktnummer ABIN3032070

Produktdetails anti-NOL3 Antikörper

Target: NOL3 (Nucleolar Protein 3 (Apoptosis Repressor with CARD Domain) (NOL3))

Bindungsspezifität: AA 91-106

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NOL3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Aufreinigung: Antigen affinity

Immunogen: Amino acids 91-106 (WDWQHVGPGYRDRSYD-human) were used as the immunogen for this ARC antibody (100% rat homology).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting amounts. Titration of the ARC antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: -20 °C

Informationen zur Lagerung: After reconstitution, the ARC antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Details zu NOL3

Target: NOL3 (Nucleolar Protein 3 (Apoptosis Repressor with CARD Domain) (NOL3))

Andere Bezeichnung: ARC Apoptosis Repressor with CARD (NOL3 Produkte)

Synonyme: ARC antikoerper, FCM antikoerper, MYP antikoerper, NOP antikoerper, NOP30 antikoerper, B430311C09Rik antikoerper, MYC antikoerper, Nop30 antikoerper, Arc antikoerper, nucleolar protein 3 antikoerper, nucleolar protein 3 (apoptosis repressor with CARD domain) antikoerper, NOL3 antikoerper, Nol3 antikoerper

Hintergrund: Apoptosis Repressor with CARD, also known as ARC, NOP30, NOL3, CARD2 and MYP, is a protein that in humans is encoded by the NOL3 gene. NOL3 has been shown to interact with SFRS9 and Caspase 8. By genomic sequence analysis, Stoss et al.(1999) determined that the NOL3 gene, which encodes NOP30 and MYP and which they called NOP, is composed of 4 exons. The alternative 5-prime splice site that generates the two isoforms is located in exon 2. It is reported that expression of the Apoptosis Repressor with CARD/ARC cDNA encoding the smaller transcript inhibited apoptosis in a dose-dependent manner when coexpressed with CASP8 but not when coexpressed with CASP9. The protein also inhibited apoptosis induced by stimulation of CD95/FAS, tumor necrosis factor receptor-1, and TRAMP/death receptor-3. Enzymatic analysis showed that ARC inhibits the enzymatic activity of CASP8. Immunoprecipitation and immunoblot analysis indicated that ARC interacts with CASP2 and -8 through its N-terminal death effector domain but does not interact with CASP1, -3, or -9.

UniProt: O60936