TdT Antikörper (AA 316-509)

Produktnummer ABIN3030715

Produktdetails anti-TdT Antikörper

Target: TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))

Bindungsspezifität: AA 316-509

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TdT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Aufreinigung: Antigen affinity

Immunogen: Human partial recombinant protein (AA 316-509) was used as the immunogen for this TdT antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting amounts. Titration of the TdT antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: -20 °C

Informationen zur Lagerung: After reconstitution, the TdT antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Details zu TdT

Target: TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))

Andere Bezeichnung: TdT (DNTT Produkte)

Synonyme: TdT antikoerper, TDT antikoerper, BB160593 antikoerper, Tdt antikoerper, dntt-A antikoerper, dntt antikoerper, DNA nucleotidylexotransferase antikoerper, deoxynucleotidyltransferase, terminal antikoerper, DNA nucleotidylexotransferase L homeolog antikoerper, terminal deoxynucleotidyl transferase antikoerper, DNTT antikoerper, dntt antikoerper, Dntt antikoerper, dntt.L antikoerper, tdt antikoerper

Hintergrund: Terminal Deoxynucleotidyl Transferase, also known as Terminal transferase, is a unique DNA polymerase that without template direction catalyzes the addition of deoxyribonucleotides onto the 3-prime-hydroxyl end of DNA primers. Its gene is mapped to the region 10q23-q24. And TdT cDNA contains an open reading frame of 1,530 basepairs corresponding to a protein containing 510 amino acids. TdT may be responsible for inserting nucleotides (N regions) at the V(H)-D and D-J(H) junctions of immunoglobulin genes. The enzyme is present in immature thymocytes, some bone marrow cells, transformed pre-B and pre-T cell lines, and leukemia cells. Additionally, TdT catalyses the addition of nucleotides to the 3' terminus of a DNA molecule. Unlike most DNA polymerases it does not require a template. The preferred substrate of this enzyme is a 3'-overhang, but it can also add nucleotides to blunt or recessed 3' ends. Cobalt is a necessary cofactor.

Gen-ID: 1791

Pathways: DNA Reparatur