COX1 Antikörper (C-Term)

Produktnummer ABIN3030663

Produktdetails anti-COX1 Antikörper

Target: COX1 (Cytochrome C Oxidase Subunit I (COX1))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser COX1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Aufreinigung: Antigen affinity

Immunogen: An amino acid sequence from the C-terminus of human MTCO1 (PYHTFEEPVYMKS) was used as the immunogen for this MTCO1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting amounts. Titration of the MTCO1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: -20 °C

Informationen zur Lagerung: After reconstitution, the MTCO1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Details zu COX1

Target: COX1 (Cytochrome C Oxidase Subunit I (COX1))

Andere Bezeichnung: MTCO1 (COX1 Produkte)

Synonyme: COX-1 antikoerper, COI antikoerper, MTCO1 antikoerper, COX1 antikoerper, CoxI antikoerper, prostaglandin-endoperoxide synthase 1 antikoerper, cytochrome c oxidase subunit I antikoerper, cytochrome c oxidase subunit 1 antikoerper, cytochrome c oxidase 1 antikoerper, PTGS1 antikoerper, COX1 antikoerper, cox1 antikoerper

Hintergrund: Cytochrome c oxidase subunit I (CO1 or MTCO1) is 1 of 3 mitochondrial DNA(mtDNA) encoded subunits (MTCO1, MTCO2, MTCO3) of respiratory Complex IV. Complex IV is located within the mitochondrial inner membrane and is the third and final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. It is composed of 13 polypeptides. Subunits I, II, and III (MTCO1, MTCO2, MTCO3) are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc, and VIII are nuclear encoded. The cytochrome c oxidase family of enzymes have 4 redox centers, 2 hemes and 2 copper centers. In mitochondrial Complex IV, the 2 hemes are a and a3 and the 2 coppers are CuA and CuB. The 2 hemes and CuB are bound to subunit I. Acin-Perez et al.(2003) identified a cell line containing single and double missense mutations in the cytochrome c oxidase (COX) subunit I gene of mouse mitochondrial DNA. And they hypothesized that deleterious mutations can arise and become predominant, cultured cells can maintain several mtDNA haplotypes at stable frequencies, the respiratory chain has little spare COX capacity, and that the size of a cavity in the vicinity of val421 in MTCO1I of animal COX may affect the function of the enzyme.

UniProt: P00395

Pathways: Proton Transport