Fascin Antikörper

Produktnummer ABIN3025905

Produktdetails anti-Fascin Antikörper

Target: Fascin (FSCN1)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Fascin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Aufreinigung: Protein G affinity chromatography

Immunogen: Full length recombinant human protein was used as the immunogen for the Fascin antibody.

Klon: FSCN1-417

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Fascin antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Fascin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu Fascin

Target: Fascin (FSCN1)

Andere Bezeichnung: Fascin (FSCN1 Produkte)

Synonyme: CG15331 antikoerper, CG1536 antikoerper, CG32858 antikoerper, Dmel\\CG32858 antikoerper, SN antikoerper, Sn antikoerper, fs(1)A1057 antikoerper, fs(1)K1421 antikoerper, fs(1)K418 antikoerper, fs(1)K473 antikoerper, fs(1)K743 antikoerper, fs(1)M45 antikoerper, p55 antikoerper, snl antikoerper, MGC75811 antikoerper, fscn1 antikoerper, sb:cb589 antikoerper, zgc:153632 antikoerper, AI663989 antikoerper, Fan1 antikoerper, fascin-1 antikoerper, Fascin antikoerper, fscn antikoerper, FAN1 antikoerper, HSN antikoerper, SNL antikoerper, fascin antikoerper, singed antikoerper, fascin actin-bundling protein 1 antikoerper, fascin actin-bundling protein 1a antikoerper, fascin actin-bundling protein 1 L homeolog antikoerper, sn antikoerper, fscn1 antikoerper, fscn1a antikoerper, Fscn1 antikoerper, fscn1.L antikoerper, FSCN1 antikoerper

Hintergrund: Recognizes a protein of 55  kDa, which is identified as fascin-1. Its actin binding ability is regulated by phosphorylation. Antibody to fascin-1 is a very sensitive marker for Reed-Sternberg cells and variants in nodular sclerosis, mixed cellularity, and lymphocyte depletion Hodgkin's disease. It is uniformly negative in lymphoid cells, plasma cells, and myeloid cells. Fascin-1 is also expressed in dendritic cells. This marker may be helpful to distinguish between Hodgkin lymphoma and non-Hodgkin lymphoma in difficult cases. Also, the lack of expression of fascin-1 in the neoplastic follicles in follicular lymphoma may be helpful in distinguishing these lymphomas from reactive follicular hyperplasia in which the number of follicular dendritic cells is normal or increased. Antibody to fascin-1 has been suggested as a prognostic marker in neuroendocrine neoplasms of the lung as well as in ovarian cancer. Fascin-1 expression may be induced by Epstein-Barr virus (EBV) infection of B cells with the possibility that viral induction of fascin in lymphoid or other cell types must also be considered in EBV-positive cases.