Nucleolin Antikörper
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- Target Alle Nucleolin (NCL) Antikörper anzeigen
- Nucleolin (NCL)
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Reaktivität
- Human, Maus
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser Nucleolin Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Aufreinigung
- Protein G affinity chromatography
- Immunogen
- Recombinant human protein was used as the immunogen for the Nucleolin antibody.
- Klon
- NPC23-2
- Isotyp
- IgG1
- Top Product
- Discover our top product NCL Primärantikörper
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- Applikationshinweise
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Titering of the Nucleolin antibody may be required for optimal performance.
1. FFPE testing requires sections to be boiled in pH 6 10 mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. IF: 1-2 μg/mL,Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Western blot: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2) - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 0.2 mg/mL
- Buffer
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store the Nucleolin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Target
- Nucleolin (NCL)
- Andere Bezeichnung
- Nucleolin (NCL Produkte)
- Synonyme
- C23 antikoerper, B530004O11Rik antikoerper, D0Nds28 antikoerper, D1Nds28 antikoerper, Nucl antikoerper, wu:fa12d03 antikoerper, zgc:152810 antikoerper, nucl antikoerper, LOC100282923 antikoerper, LOC100283058 antikoerper, nucleolin antikoerper, nucleolin S homeolog antikoerper, NCL antikoerper, Ncl antikoerper, ncl.S antikoerper, ncl antikoerper, LOC100195537 antikoerper, LOC100282923 antikoerper, LOC100283058 antikoerper
- Hintergrund
- Nucleolin (NCL) is the major nucleolar phosphoprotein of growing eukaryotic cells. It is located mainly in dense fibrillar regions of the nucleolus. NCL is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.
- Pathways
- Ribonucleoprotein Complex Subunit Organization
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