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Nucleolin Antikörper

NCL Reaktivität: Human WB, IF, FACS, IHC (p) Wirt: Maus Monoclonal 364-5-NCL-902 unconjugated
Produktnummer ABIN3025715
  • Target Alle Nucleolin (NCL) Antikörper anzeigen
    Nucleolin (NCL)
    Reaktivität
    • 128
    • 31
    • 29
    • 10
    • 9
    • 8
    • 8
    • 8
    • 6
    • 5
    • 4
    • 4
    • 1
    • 1
    • 1
    Human
    Wirt
    • 88
    • 43
    • 1
    Maus
    Klonalität
    • 86
    • 46
    Monoklonal
    Konjugat
    • 63
    • 12
    • 8
    • 7
    • 6
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Nucleolin Antikörper ist unkonjugiert
    Applikation
    • 103
    • 61
    • 58
    • 43
    • 42
    • 19
    • 16
    • 15
    • 14
    • 14
    • 13
    • 10
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Keine Kreuzreaktivität
    Maus, Ratte (Rattus), Rind (Kuh)
    Aufreinigung
    Protein G affinity chromatography
    Immunogen
    SUDHL1 cell nuclear lysate (364-5) and recombinant human protein (NCL/902) were used as the immunogen for the Nucleolin antibody cocktail.
    Klon
    364-5-NCL-902
    Isotyp
    IgG
    Top Product
    Discover our top product NCL Primärantikörper
  • Applikationshinweise
    Optimal dilution of the Nucleolin antibody cocktail should be determined by the researcher.

    1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    0.2 mg/mL
    Buffer
    0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store the Nucleolin antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target
    Nucleolin (NCL)
    Andere Bezeichnung
    Nucleolin (NCL Produkte)
    Synonyme
    C23 antikoerper, B530004O11Rik antikoerper, D0Nds28 antikoerper, D1Nds28 antikoerper, Nucl antikoerper, wu:fa12d03 antikoerper, zgc:152810 antikoerper, nucl antikoerper, LOC100282923 antikoerper, LOC100283058 antikoerper, nucleolin antikoerper, nucleolin S homeolog antikoerper, NCL antikoerper, Ncl antikoerper, ncl.S antikoerper, ncl antikoerper, LOC100195537 antikoerper, LOC100282923 antikoerper, LOC100283058 antikoerper
    Hintergrund
    Recognizes a protein of ~76  kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This mAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
    Pathways
    Ribonucleoprotein Complex Subunit Organization
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