MAML2 Antikörper

Produktnummer ABIN3025218

Produktdetails anti-MAML2 Antikörper

Target: MAML2 (Mastermind-Like 2 (MAML2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MAML2 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Aufreinigung: Protein G affinity chromatography

Immunogen: A human recombinant protein was used as the immunogen for this MAML2 antibody.

Klon: MMLP2-1

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MAML2 antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissue requires boiling sections in 10  mM Tris with 1  mM EDTA,  pH 9, for 10-20 min followed by cooling at RT for 20 min.\. FACS: 0.5-1 μg/10^6 cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the MAML2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu MAML2

Target: MAML2 (Mastermind-Like 2 (MAML2))

Andere Bezeichnung: MAML2 (MAML2 Produkte)

Synonyme: 5930431H10 antikoerper, BC032967 antikoerper, MAM-3 antikoerper, MAM2 antikoerper, MAM3 antikoerper, MLL-MAML2 antikoerper, mastermind like 2 (Drosophila) antikoerper, mastermind-like transcriptional coactivator 2 antikoerper, mastermind like transcriptional coactivator 2 antikoerper, Maml2 antikoerper, MAML2 antikoerper

Hintergrund: Mastermind-like 2, also known as MAM2, MAM3 or MLLMAML2, is a nuclear speckle protein that acts as a transcriptional co-activator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML2 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, it interacts with the ankyrin repeats of the Notch proteins (1-4). This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-Jκ, a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML2 is essential for proper Notch binding, the C-terminal domain of MAML2 is essential for transcriptional activation. A chromosomal aberration involving the gene is implicated in mucoepidermoid carcinomas, clear cell hidradenomas and benign Warthin tumors.

Gen-ID: 84441