CD31 Antikörper

Produktnummer ABIN3024342

Produktdetails anti-CD31 Antikörper

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reaktivität: Human, Kaninchen, Cynomolgus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD31 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Keine Kreuzreaktivität: Ratte (Rattus), Schwein

Aufreinigung: Protein G affinity chromatography

Immunogen: A membrane preparation of a spleen from a patient with hairy cell leukemia was used as the immunogen for this CD31 antibody.

Klon: JC-70A

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: The optimal dilution of the CD31 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 minutes at RT (1),Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the CD31 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Referenzen für anti-CD31 Antikörper (ABIN3024342)

Shi, Liang, Guo, Zhang, Hui, Chang, Tao: "Adipose-Derived Stem Cells Cocultured with Chondrocytes Promote the Proliferation of Chondrocytes." in: Stem cells international, Vol. 2017, pp. 1709582, (2017) (PubMed).

Details zu CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Andere Bezeichnung: CD31 (PECAM1 Produkte)

Synonyme: CD31 antikoerper, CD31/EndoCAM antikoerper, GPIIA' antikoerper, PECA1 antikoerper, PECAM-1 antikoerper, endoCAM antikoerper, C85791 antikoerper, Cd31 antikoerper, Pecam antikoerper, platelet/endothelial cell adhesion molecule 1 antikoerper, platelet and endothelial cell adhesion molecule 1 antikoerper, pecam1 antikoerper, PECAM1 antikoerper, Pecam1 antikoerper

Hintergrund: CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 mAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.

Pathways: Regulation of Actin Filament Polymerization