RBPJ Antikörper (AA 208-500)
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- Target Alle RBPJ Antikörper anzeigen
- RBPJ (Recombination Signal Binding Protein For Immunoglobulin kappa J Region (RBPJ))
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Bindungsspezifität
- AA 208-500
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Reaktivität
- Human
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Dieser RBPJ Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
- Spezifität
- Selected for its ability to recognize human CSL in the applications listed.
- Aufreinigung
- Affinity purified
- Immunogen
- E. coli-derived rhCSL (aa208-500).
- Isotyp
- IgG
- Top Product
- Discover our top product RBPJ Primärantikörper
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- Applikationshinweise
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Approved: ELISA, ICC, WB
Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human CSL. The detection limit for rhCSL is approximately 10 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: This antibody can be used at a concentration of 10 μg/mL to detect human CSL on HASMC (human aortic smooth muscle) cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by NorthernLights 557-coupled donkey anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human CSL. The detection limit for rhCSL is approximately 5 ng/well. - Kommentare
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Target Species of Antibody: Human
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- deionized water
- Konzentration
- Lot specific
- Buffer
- Lyophilized.
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- Target
- RBPJ (Recombination Signal Binding Protein For Immunoglobulin kappa J Region (RBPJ))
- Andere Bezeichnung
- RBPJ (RBPJ Produkte)
- Synonyme
- AOS3 antikoerper, CBF1 antikoerper, IGKJRB antikoerper, IGKJRB1 antikoerper, KBF2 antikoerper, RBP-J antikoerper, RBPJK antikoerper, RBPSUH antikoerper, SUH antikoerper, csl antikoerper, AI843960 antikoerper, Igkjrb antikoerper, Igkrsbp antikoerper, RBP-J kappa antikoerper, RBPjk antikoerper, Rbpsuh antikoerper, Su(H) antikoerper, XSu(H) antikoerper, xsu(h)1 antikoerper, Su(H)1 antikoerper, Su(h)A antikoerper, ik:tdsubc_1e8 antikoerper, rbpj antikoerper, rbpsuh antikoerper, xx:tdsubc_1e8 antikoerper, Su(H)2 antikoerper, su(h)B antikoerper, recombination signal binding protein for immunoglobulin kappa J region antikoerper, recombination signal binding protein for immunoglobulin kappa J region S homeolog antikoerper, recombination signal binding protein for immunoglobulin kappa J region a antikoerper, recombination signal binding protein for immunoglobulin kappa J region b antikoerper, RBPJ antikoerper, Rbpj antikoerper, rbpj.S antikoerper, rbpja antikoerper, rbpjb antikoerper
- Hintergrund
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Name/Gene ID: RBPJ
Synonyms: RBPJ, AOS3, CBF1, Csl, KBF2, H-2K binding factor-2, RBP-JK, SUH, Suppressor of hairless homolog, RBP-J, RBP-J kappa, RBPJK, RBPSUH, CBF-1, IGKJRB, IGKJRB1 - Gen-ID
- 3516
- UniProt
- Q06330
- Pathways
- Notch Signalweg, Stem Cell Maintenance, Smooth Muscle Cell Migration
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