ARPC2 Antikörper
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- Target Alle ARPC2 Antikörper anzeigen
- ARPC2 (Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2))
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Reaktivität
- Human
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser ARPC2 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Kreuzreaktivität
- Human, Maus, Ratte, Zebrafisch (Danio rerio)
- Produktmerkmale
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Rabbit Polyclonal antibody to ARPC2 (actin related protein 2/3 complex, subunit 2, 34 kDa)
ARPC2 antibody - Aufreinigung
- Purified by antigen-affinity chromatography.
- Immunogen
- Recombinant protein encompassing a sequence within the center region of human ARPC2. The exact sequence is proprietary.
- Isotyp
- IgG
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- Applikationshinweise
- WB: 1:500-1:5000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
- Kommentare
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Positive Control: rat kidney , mouse kidney
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- by
- Akoya Biosciences
- No.
- #104342
- Datum
- 20.04.2021
- Antigen
- ARPC2
- Chargennummer
- 39911
- Validierte Anwendung
- Multiplex Immunohistochemistry
- Positivkontrolle
FFPE normal human cortex
- Negativkontrolle
Unlabeled control (FFPE normal human cortex)
- Bewertung
Passed. The anti-ARPC2 antibody ABIN2855249 produced staining in neuronal processes throughout the human cortex.
- Primärantikörper
- ABIN2855249
- Sekundärantikörper
- Full Protocol
- Protocol details are described in the Akoya Biosciences CODEX® User Manual (see https://www.akoyabio.com/wp-content/uploads/2021/01/CODEX-User-Manual.pdf).
- Tissue preparation as outlined in the Akoya Biosciences CODEX® User Manual fresh-frozen tissue protocol.
- Conjugation of the anti-ARPC2 antibody ABIN2855249 to an oligo barcode used to bind oligo-conjugated fluorophore AF488.
- Anmerkungen
No signal was detected in unlabeled specimens.
Specific staining of ARPC2 was observed with human FFPE cortical tissue sections with tris-EDTA antigen retrieval.
Anti-ARPC antibody ABIN2855249 was weakly compatible with staining of fresh frozen mouse tissue
- Optimal staining and signal to noise ratios were obtained if tissue was pre-treated for autofluorescence removal (see https://www.akoyabio.com/wp-content/uploads/2020/07/Customer-Demonstrated-Protocol-Autofluorescence-Quenching-Mar2020.pdf).
Validierung #104342 (Multiplex Immunohistochemistry)ValidierungsbilderFFPE normal human cortex tissue section labeled with anti-ARPC2 antibody ABIN2855249 (cyan; conjugated to fluorophore AF488) after EDTA antigen retrieval. MAP2, neurofilament, and GFAP were labeled with anti-MAP2 antibody ABIN125739 (green; conjugated to fluorophore ATTO 550), anti-neurofilament antibody (red; conjugated to fluorophore AF488), and anti-GFAP antibody (purple; conjugated to fluorophore AF647) respectively.Protokoll -
- Format
- Liquid
- Konzentration
- 1 mg/mL
- Buffer
- 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300
- Konservierungsmittel
- ProClin
- Vorsichtsmaßnahmen
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- Target
- ARPC2 (Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2))
- Andere Bezeichnung
- actin related protein 2/3 complex subunit 2 (ARPC2 Produkte)
- Synonyme
- arc34 antikoerper, p34-arc antikoerper, pnas-139 antikoerper, pro2446 antikoerper, Arpc2 antikoerper, Arc-p34 antikoerper, 2210023N03Rik antikoerper, 34kDa antikoerper, p34-Arc antikoerper, fk84a07 antikoerper, wu:fa22f07 antikoerper, wu:fk84a07 antikoerper, zgc:77769 antikoerper, ARC34 antikoerper, PNAS-139 antikoerper, actin related protein 2/3 complex subunit 2 antikoerper, actin related protein 2/3 complex, subunit 2, 34kDa antikoerper, actin related protein 2/3 complex, subunit 2 antikoerper, actin related protein 2/3 complex subunit 2 S homeolog antikoerper, ARPC2 antikoerper, arpc2 antikoerper, Arpc2 antikoerper, arpc2.S antikoerper
- Hintergrund
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This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p34 subunit, has yet to be determined. Two alternatively spliced variants have been characterized to date. Additional alternatively spliced variants have been described but their full length nature has not been determined.
Cellular Localization: Cytoplasm , cytoskeleton , Cell projection - Molekulargewicht
- 34 kDa
- Gen-ID
- 10109
- UniProt
- O15144
- Pathways
- RTK Signalweg, Regulation of Actin Filament Polymerization
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