GTF3C2 Antikörper

Produktnummer ABIN272244

Produktdetails anti-GTF3C2 Antikörper

Target: GTF3C2 (General Transcription Factor IIIC, Polypeptide 2, beta 110kDa (GTF3C2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GTF3C2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: This antibody detects endogenous levels of TFIIIC110 protein. (region surrounding Arg95)

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Reinheit: > 95 % pure by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: DO NOT FREEZE!

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Details zu GTF3C2

Target: GTF3C2 (General Transcription Factor IIIC, Polypeptide 2, beta 110kDa (GTF3C2))

Andere Bezeichnung: GTF3C2 / TFIIIC110 (GTF3C2 Produkte)

Synonyme: TFIIIC110 antikoerper, GTF3C2 antikoerper, MGC155253 antikoerper, TF3C-beta antikoerper, DKFZp469M0113 antikoerper, 1300004C11Rik antikoerper, 2610510G03Rik antikoerper, AI225816 antikoerper, AU041069 antikoerper, mKIAA0011 antikoerper, TFIIIC-BETA antikoerper, general transcription factor IIIC subunit 2 antikoerper, general transcription factor IIIC, polypeptide 2, beta antikoerper, Gtf3c2 antikoerper, GTF3C2 antikoerper

Hintergrund: RNA polymerase (pol) III synthesizes tRNA, 5s rRNA, 7SL RNA and U6 snRNA and is overexpressed in many transformed cell lines and tumors in vivo, since cells must duplicate its protein components before division. Therefore, in order to maintain rapid growth, cells must produce a high level of Pol III transcribed RNA, which requires the presence of the TFIIIB and TFIIIC2 transcription factor complexes. The TFIIIC2 complex is composed of five subunits, TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90 and TFIIIC63, that are overexpressed in adenovirus transformed cells as well as in malignant cells in vivo, such as ovarian carcinomas. TFIIIC2 recruits RNA pol III and TFIIIB to promoter elements and may be a key component in the deregulation of malignant cells. The TFIIIB complex includes the TATA-binding protein (TBP), TFIIB-related factor 1 (BRF1) and TFIIIB", the expression of which are also upregulated in transformed cells. In many carcinomas, the tumor suppressors retinoblastoma (RB) and p53 are inactivated, which affects their ability to bind and inactivate the function of TFIIIB.Synonyms: General transcription factor 3C polypeptide 2, KIAA0011, TF3C-beta, Transcription factor IIIC 110 kDa subunit, Transcription factor IIIC subunit beta

Molekulargewicht: approx. 100 kDa

Gen-ID: 2976

NCBI Accession: NP_001030598

UniProt: Q8WUA4