RHOA Antikörper

Produktnummer ABIN272117

Produktdetails anti-RHOA Antikörper

Target: RHOA (Ras Homolog Gene Family, Member A (RHOA))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RHOA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: This antibody detects endogenous levels of Rho A protein. (region surrounding Arg182)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Reinheit: > 95 % pure by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: DO NOT FREEZE!

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Details zu RHOA

Target: RHOA (Ras Homolog Gene Family, Member A (RHOA))

Andere Bezeichnung: RhoA (RHOA Produkte)

Synonyme: ARH12 antikoerper, ARHA antikoerper, RHO12 antikoerper, RHOH12 antikoerper, Arha antikoerper, Arha1 antikoerper, Arha2 antikoerper, arha antikoerper, arha2 antikoerper, xrhoa antikoerper, arh12 antikoerper, rho12 antikoerper, rhoh12 antikoerper, rhoa antikoerper, RHO1 antikoerper, Rhoa antikoerper, wu:fa66g04 antikoerper, wu:fb26g07 antikoerper, zgc:66058 antikoerper, zgc:85805 antikoerper, ARHC antikoerper, rhoae antikoerper, zgc:64015 antikoerper, ras homolog family member A antikoerper, ras homolog family member A S homeolog antikoerper, ras homolog family member A L homeolog antikoerper, ras homolog gene family, member Aa antikoerper, ras homolog family member Ca antikoerper, RHOA antikoerper, Rhoa antikoerper, rhoa.S antikoerper, rhoa antikoerper, rhoa.L antikoerper, rhoaa antikoerper, rhoca antikoerper

Hintergrund: The Ras p21 family of guanine nucleotide proteins has been widely studied in view of its apparent role in signal transduction pathways and high frequency of mutations in human malignancies. It is now clear, however, that the Ras proteins (H-, K- and N-Ras p21) are members of a much larger superfamily of related proteins. Six members of this family, Rap 1 (A and B), Rap 2, R-Ras and Ral (A and B), exhibit approximately 50 % amino acid homology to Ras. The three mammalian Rho proteins (A, B and C) are approximately 30 % homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy terminal CAAX sequence (C, cysteine, A, aliphatic amino acid, X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function.Synonyms: ARH12, ARHA, RHO12, Rho cDNA clone 12, Transforming protein RhoA

Molekulargewicht: approx. 22 kDa

Gen-ID: 387

NCBI Accession: NP_001655

UniProt: P61586

Pathways: Microtubule Dynamics, WNT Signalweg, Neurotrophin Signalübertragung, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, SARS-CoV-2 Protein Interaktom