YAP1 Antikörper

Produktnummer ABIN272105

Produktdetails anti-YAP1 Antikörper

Target: YAP1 (Yes-Associated Protein 1 (YAP1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser YAP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: This antibody detects endogenous levels of YAP protein. (region surrounding His122)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Reinheit: > 95 % pure by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: DO NOT FREEZE!

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Details zu YAP1

Target: YAP1 (Yes-Associated Protein 1 (YAP1))

Andere Bezeichnung: YAP1 (YAP1 Produkte)

Synonyme: YAP antikoerper, YAP2 antikoerper, YAP65 antikoerper, YKI antikoerper, xyap antikoerper, yap antikoerper, yap2 antikoerper, yap65 antikoerper, yki antikoerper, AI325207 antikoerper, Yap antikoerper, Yap65 antikoerper, Yki antikoerper, Yorkie antikoerper, cb194 antikoerper, sb:cb194 antikoerper, si:ch211-181p1.5 antikoerper, si:dkey-3b8.3 antikoerper, wu:fc18c04 antikoerper, zgc:158380 antikoerper, Yes associated protein 1 antikoerper, Yes associated protein 1 S homeolog antikoerper, yes-associated protein 1 antikoerper, Yes-associated protein 1 antikoerper, YAP1 antikoerper, yap1.S antikoerper, Yap1 antikoerper, yap1 antikoerper

Hintergrund: The Yes-associated protein, otherwise known as YAP, is a 14-3-3-binding molecule that was originally recognized by virtue of its ability to bind to the SH3 domain of Yes. The binding of YAP to 14-3-3 requires the phosphorylation of a homologous serine residue (Ser 112) in the YAP 14-3-3-binding motif. The highly conserved and ubiquitously expressed 14-3-3 proteins regulate differentiation, cell cycle progression and apoptosis by binding intracellular phosphoproteins involved in signal transduction. YAP may link events at the plasma membrane and cytoskeleton to inhibition of transcription in the nucleus in a manner regulated by 14-3-3 proteins. YAP shares homology with the WW domain of TAZ, transcriptional co-activator with PDZ-binding motif, which functions as a transcriptional co-activator by binding to the PPXY motif present in transcription factors. YAP is expressed at high levels in the lung, placenta, prostate, ovary and testis.Synonyms: 65 kDa Yes-associated protein, YAP, YAP65

Molekulargewicht: approx. 38, 67 kDa

Gen-ID: 10413

NCBI Accession: NP_001123617

UniProt: P46937

Pathways: MAPK Signalweg, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha