FAS Antikörper

Produktnummer ABIN2689460

Produktdetails anti-FAS Antikörper

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Reaktivität: Human, Schwein, Rhesusaffen, Hund, Pavian, Cynomolgus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FAS Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Marke: BD Pharmingen™

Produktmerkmale: Reacts with the 45 kDa transmembrane cell surface Fas or Apo-1 antigen (designated CD95 at the Fifth HLDA Workshop) which is expressed on a variety of normal and neoplastic cells. The Fas/Apo-1 antigen is a polypeptide, which plays a role in the programmed sequence of events leading to cell death, termed apoptosis. The DX2 clone specifically reacts with murine L cells, murine L1210 leukemia cells and murine P815 mastocytoma cells transfected with human Fas cDNA but not with untransfected parental cell lines. Cross-linking with DX2 delivers an apoptotic signal indicating that DX2 recognizes a functional epitope of the CD95 antigen. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of peripheral blood lymphocytes analyzed by flow cytometry DX2-induced apoptosis in Fas transfectant cells line P825/FASDT151 detected by DNA fragmentation analysis. Cells were incubated alone (lane 1), in the presence of mouse IgG1 (lane 2) or in the presence of DX2 at 10, 100 or 500 ng/mL (lanes 3, 4 and 5, respectively).

BD Pharmingen™ Purified Mouse Anti-Human CD95 - Purified - Clone DX2 - Isotype Mouse IgG1, κ - Reactivity Hu, Bab, Cyno, Dog, Pig, Rhe - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Klon: DX2

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-FAS Antikörper (ABIN2689460)

Cifone, De Maria, Roncaioli, Rippo, Azuma, Lanier, Santoni, Testi: "Apoptotic signaling through CD95 (Fas/Apo-1) activates an acidic sphingomyelinase." in: The Journal of experimental medicine, Vol. 180, Issue 4, pp. 1547-52, (1994) (PubMed).

Itoh, Yonehara, Ishii, Yonehara, Mizushima, Sameshima, Hase, Seto, Nagata: "The polypeptide encoded by the cDNA for human cell surface antigen Fas can mediate apoptosis." in: Cell, Vol. 66, Issue 2, pp. 233-43, (1991) (PubMed).

Details zu FAS

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Andere Bezeichnung: CD95 (FAS Produkte)

Synonyme: ALPS1A antikoerper, APO-1 antikoerper, APT1 antikoerper, CD95 antikoerper, FAS1 antikoerper, FASTM antikoerper, TNFRSF6 antikoerper, AI196731 antikoerper, APO1 antikoerper, TNFR6 antikoerper, Tnfrsf6 antikoerper, lpr antikoerper, Fas cell surface death receptor antikoerper, Fas (TNF receptor superfamily member 6) antikoerper, FAS antikoerper, Fas antikoerper, fas antikoerper

Hintergrund: Synonyms: Fas/APO-1

Pathways: p53 Signalweg, Apoptose, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity