CD31 Antikörper (Biotin)

Produktnummer ABIN2689177

Produktdetails anti-CD31 Antikörper (Biotin)

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD31 Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS)

Marke: BD Pharmingen™

Produktmerkmale: The 390 monoclonal antibody specifically binds to CD31, also known as PECAM-1 (platelet endothelial cell adhesion molecule). CD31 is a ~130 kDa integral membrane glycoprotein, a member of the immunoglobulin superfamily, that mediates homophilic and heterophilic cell-cell adhesion. CD31 is expressed constitutively on the surface of adult and embryonic endothelial cells and is weakly expressed on many peripheral leukocytes and platelets. It has also been detected on bone marrow-derived hematopoietic stem cells and embryonic stem cells. CD31 is involved in the transendothelial emigration of neutrophils, and neutrophil PECAM-1 appears to be down-regulated after extravasation into inflamed tissues. Multiple alternatively spliced isoforms are detected during early post-implantation embryonic development, this alternative splicing is involved in regulation of ligand specificity. CD38 and vitronectin receptor (αvβ3 integrin, CD51/CD61) are proposed to be ligands for CD31. CD31-mediated endothelial cell-cell interactions are involved in angiogenesis. The 390 mAb inhibits a variety of in vitro and in vivo functions mediated by CD31.

BD Pharmingen™ Biotin Rat Anti-Mouse CD31 - Biotin - Clone 390 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: C3H/HeJ mouse hematopoietic progenitor cell line 32D

Klon: 390

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Referenzen für anti-CD31 Antikörper (Biotin) (ABIN2689177)

Duncan, Andrew, Takimoto, Kaufman, Yoshida, Spellberg, de la Pompa, Elia, Wakeham, Karan-Tamir, Muller, Senaldi, Zukowski, Mak: "Genetic evidence for functional redundancy of Platelet/Endothelial cell adhesion molecule-1 (PECAM-1): CD31-deficient mice reveal PECAM-1-dependent and PECAM-1-independent functions." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 5, pp. 3022-30, (1999) (PubMed).

Horenstein, Stockinger, Imhof, Malavasi: "CD38 binding to human myeloid cells is mediated by mouse and human CD31." in: The Biochemical journal, Vol. 330 ( Pt 3), pp. 1129-35, (1998) (PubMed).

Buck, Edelman, Buck, Kennedy, Baldwin: "Expression patterns of adhesion receptors in the developing mouse lung: functional implications." in: Cell adhesion and communication, Vol. 4, Issue 2, pp. 69-87, (1997) (PubMed).

DeLisser, Christofidou-Solomidou, Strieter, Burdick, Robinson, Wexler, Kerr, Garlanda, Merwin, Madri, Albelda: "Involvement of endothelial PECAM-1/CD31 in angiogenesis." in: The American journal of pathology, Vol. 151, Issue 3, pp. 671-7, (1997) (PubMed).

Famiglietti, Sun, DeLisser, Albelda: "Tyrosine residue in exon 14 of the cytoplasmic domain of platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) regulates ligand binding specificity." in: The Journal of cell biology, Vol. 138, Issue 6, pp. 1425-35, (1997) (PubMed).

Iguchi, Okuyama, Koguma, Obinata, Yanai: "Selective stimulation of granulopoiesis in vitro by established bone marrow stromal cells." in: Cell structure and function, Vol. 22, Issue 3, pp. 357-64, (1997) (PubMed).

Ling, Luxenberg, Wang, Nickbarg, Leenen, Neben, Kobayashi: "Structural identification of the hematopoietic progenitor antigen ER-MP12 as the vascular endothelial adhesion molecule PECAM-1 (CD31)." in: European journal of immunology, Vol. 27, Issue 2, pp. 509-14, (1997) (PubMed).

Rosenblum, Nelson, Wormley, Werner, Wang, Shih: "Role of platelet-endothelial cell adhesion molecule (PECAM) in platelet adhesion/aggregation over injured but not denuded endothelium in vivo and ex vivo." in: Stroke; a journal of cerebral circulation, Vol. 27, Issue 4, pp. 709-11, (1996) (PubMed).

Piali, Hammel, Uherek, Bachmann, Gisler, Dunon, Imhof: "CD31/PECAM-1 is a ligand for alpha v beta 3 integrin involved in adhesion of leukocytes to endothelium." in: The Journal of cell biology, Vol. 130, Issue 2, pp. 451-60, (1995) (PubMed).

Buck, Baldwin, DeLisser, Mickanin, Shen, Kennedy, Chen, Edelman, Albelda: "Cell adhesion receptors and early mammalian heart development: an overview." in: Comptes rendus de l'Acade?mie des sciences. Se?rie III, Sciences de la vie, Vol. 316, Issue 9, pp. 838-59, (1994) (PubMed).

DeLisser, Newman, Albelda: "Molecular and functional aspects of PECAM-1/CD31." in: Immunology today, Vol. 15, Issue 10, pp. 490-5, (1994) (PubMed).

Details zu CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Andere Bezeichnung: CD31 (PECAM1 Produkte)

Synonyme: CD31 antikoerper, CD31/EndoCAM antikoerper, GPIIA' antikoerper, PECA1 antikoerper, PECAM-1 antikoerper, endoCAM antikoerper, C85791 antikoerper, Cd31 antikoerper, Pecam antikoerper, platelet/endothelial cell adhesion molecule 1 antikoerper, platelet and endothelial cell adhesion molecule 1 antikoerper, pecam1 antikoerper, PECAM1 antikoerper, Pecam1 antikoerper

Hintergrund: Synonyms: Platelet endothelial cell adhesion molecule, Pecam1, PECAM-1, Pecam-1

Pathways: Regulation of Actin Filament Polymerization