GAD Antikörper

Produktnummer ABIN265428

Produktdetails anti-GAD Antikörper

Target: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GAD Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: The antibody detects endogenous levels of GAD67 protein. (region surrounding Ala492)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Reinheit: > 95 % pure by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 10000approx. 1: 20000. Western blot: 1: 500approx. 1: 1000. Immunohistochemistry: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu GAD

Target: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Andere Bezeichnung: GAD1 / GAD67 (GAD1 Produkte)

Synonyme: CPSQ1 antikoerper, GAD antikoerper, SCP antikoerper, GAD-67 antikoerper, cpsq1 antikoerper, gad1 antikoerper, gad1-A antikoerper, gad67 antikoerper, GAD67 antikoerper, GAD1 antikoerper, GLUTAMATE DECARBOXYLASE antikoerper, GLUTAMATE DECARBOXYLASE 1 antikoerper, MKP11.30 antikoerper, MKP11_30 antikoerper, glutamate decarboxylase antikoerper, EP10 antikoerper, GAD25 antikoerper, GAD44 antikoerper, Gad-1 antikoerper, Gad67 antikoerper, glutamate decarboxylase 1 antikoerper, glutamate decarboxylase 1 L homeolog antikoerper, glutamate decarboxylase antikoerper, glutamate decarboxylase 1b antikoerper, GAD1 antikoerper, gad1.1.L antikoerper, GAD antikoerper, Gad1 antikoerper, gad1b antikoerper

Hintergrund: GAD-65 and GAD-67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate limiting step in the production of GABA (γ-aminobutyric acid) from L-glutamic acid. Although both GADs are found in the brain, GAD-65 localizes to synaptic vesicle membranes in nerve terminals, while GAD-67 is distributed throughout the cell. GAD-67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD-65 will transiently activate to assist in GABA production. The loss of GAD-65 is detrimental and can impair GABA neurotransmission, however the loss of GAD-67 is lethal. Due to alternative splicing, two isoforms exist for GAD-67: the predominant GAD-67 form and the minor GAD-25 form.Synonyms: 67 kDa glutamic acid decarboxylase, GAD-67, Glutamate decarboxylase 1, Glutamate decarboxylase 67 kDa isoform

Molekulargewicht: approx. 67 kDa

Gen-ID: 2571

NCBI Accession: NP_000808

UniProt: Q99259