EEF2K Antikörper

Produktnummer ABIN265393

Produktdetails anti-EEF2K Antikörper

Target: EEF2K (Eukaryotic Elongation Factor-2 Kinase (EEF2K))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EEF2K Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: This antibody detects endogenous levels of EEF-2 kinase / eEF2K protein (region surrounding Pro360).

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen

Reinheit: > 95 % pure by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 5000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu EEF2K

Target: EEF2K (Eukaryotic Elongation Factor-2 Kinase (EEF2K))

Andere Bezeichnung: EEF-2 Kinase (EEF2K Produkte)

Synonyme: HSU93850 antikoerper, eEF-2K antikoerper, C86191 antikoerper, SMEF2K antikoerper, eEF-2 kinase antikoerper, cb365 antikoerper, fa04b08 antikoerper, wu:fa04b08 antikoerper, zgc:100973 antikoerper, eukaryotic elongation factor 2 kinase antikoerper, eukaryotic elongation factor-2 kinase antikoerper, EEF2K antikoerper, Eef2k antikoerper, eef2k antikoerper, LOC101930123 antikoerper

Hintergrund: The activity of the purified eukaryotic elongation-factor-2 kinase (eEF2K) is completely dependent on calcium and calmodulin, and autophosphorylation on serine and threonine residues is calcium/calmodulin-dependent. eEF2K is a ubiquitous protein kinase that phosphorylates and inactivates eEF2, and thus can modulate the rate of polypeptide chain elongation during translation. eEF2K is 120 kDa protein that is detected in skeletal muscle extracts and is phosphorylated rapidly by SAPK4, but poorly by p38, p38g, JNK or ERK 2. SAPK4 phosphorylates eEF2K at Ser 359 and Ser 396 in vitro, causing its inactivation. The phosphorylation of eEF2K at Ser 359 is also induced by insulin-like growth factor-1. Ser 359 is in close proximity to Ser 366 and the Ser 366 residue also becomes phosphorylated in response to growth factors. eEF2K is phosphorylated by p70 S6 kinase at Ser 366 and this results in the inactivation of eEF2K, especially at low (micromolar) calcium concentrations.Synonyms: Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase, EEF2K, EF2K, Elongation factor 2 kinase

Molekulargewicht: approx. 105 kDa

Gen-ID: 29904

NCBI Accession: NP_037434

UniProt: O00418

Pathways: AMPK Signaling