C-JUN Antikörper

Produktnummer ABIN265354

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch C-JUN in WB und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für C-JUN Antikörper (ABIN265354)

Target: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser C-JUN Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-C-JUN Antikörper

Spezifität: Antibody detects endogenous levels of AP-1 / c-Jun protein (region surrounding Asn57).

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Reinheit: > 95 % pure by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 5000-1: 10000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu C-JUN

Target: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Andere Bezeichnung: AP-1 / C-Jun

Hintergrund: Genes belonging to the Jun and Fos oncogene families encode nuclear proteins that are found to be associated with a number of transcriptional complexes. The c-Jun protein is a major component of the transcription factor AP-1, originally shown to mediate phorbol ester tumor promoter (TPA)-induced expression of responsive genes through the TPA- response element (TRE). The Jun proteins form homo- and heterodimers which bind the TRE, while Fos proteins are active only as heterodimers with any of the Jun proteins. Fos/Jun heterodimers have a much higher affinity for the TRE than Jun homodimers. Ha-Ras augments c-Jun activity and stimulates phosphorylation of its activation domain. An inhibitor of Fos/Jun function, termed IP-1, associates with Fos and Jun and is inactivated upon phosphorylation induced by the cAMP-dependent protein kinase A (PKA).Synonyms: Activator protein 1, Proto-oncogene c-jun, Transcription factor AP1, V-jun avian sarcoma virus 17 oncogene homolog, p39

Molekulargewicht: approx. 43, 48 kDa

Gen-ID: 3725

NCBI Accession: NP_002219

UniProt: P05412

Pathways: MAPK Signalweg, RTK Signalweg, WNT Signalweg, Fc-epsilon Rezeptor Signalübertragung, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Autophagie, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling, S100 Proteine