PsbO Antikörper

Produktnummer ABIN2559392

Produktdetails

Target: PsbO

Reaktivität: Spinach, Arabidopsis thaliana, Gerste, Chlamydomonas reinhardtii

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB)

Homologie: dicots including: Nicotiana tabacum, Solanum lycopersicum, trees: Populus trichocarpa

Produktmerkmale: Expected / apparent Molecular Weight of the Antigene: 35 / 33 kDa

Aufreinigung: affinity purified

Immunogen: native purified 33 kDa protein from Spinacia oleracea UniProt P12359

Anwendungsinformationen

Applikationshinweise: Recommended Dilution 1 : 2000 - 1 : 5000 with standard ECL (WB).

Kommentare:

This antibody can be used as a loading control for Chlamydomonas reinhardtii while it not so suitable for higher plants as accumulation of these proteins might drop to 12.5-25 % of the WT level in mutants defective for PSII core (Schult et al. 2007).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS pH 7.4

Handhabung: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Lagerung: -20 °C

Referenzen

Lin, Liu, Kuo, Tsai, Feng, Huang, Chien: "Knockdown of PsbO leads to induction of HydA and production of photobiological H2 in the green alga Chlorella sp. DT." in: Bioresource technology, Vol. 143, pp. 154-62, (2013) (PubMed).

Antigendetails

Target: PsbO

Hintergrund: PSII reaction centre components are generating the redox potential required to drive highly oxidizing water splitting reaction. Four Mn atoms are present on a lumenal surface and form the catalyctic site of the water-splitting reaction which is in close association with the 33 kDa (PsbO), 23 kDa (PsbP) and 17 kDa (PsbQ) extrinistic subunits of oxygen evolving complex OEC. A 33-kDa extrinsic protein is also termed the Mn-stabilizing protein (MSP), however recent evidences shown that it is C-terminal domain of PsbA (D1) protein which is involved in in the assembly and stabilization of the OEC.

Molekulargewicht: expected: 35 kDa, apparent: 33 kDa