PsbO Antikörper

Produktnummer ABIN93573

Produktdetails

Target: PsbO

Reaktivität: Arabidopsis thaliana, Gerste, Nicotiana tabacum

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Kreuzreaktivität (Details): No cross-reactivity with: Chlamydomonas reinhardtii, Synechococcus

Produktmerkmale: Expected / apparent Molecular Weight of the Antigene: 33 kDa

Aufreinigung: serum

Immunogen: N-terminually located peptide chosen from Arabidopsis thaliana PsbO1 At5g66570 and 2 proteins At5g50820

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: 1 : 1000 with standard ECL (WB).
Loading based on 50 - 100 ng of chlorophyll is enough to obtain good signal withthis antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 100 µL, of sterile water.

Handhabung: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Lagerung: -20 °C

Referenzen

Albus, Salinas, Czarnecki, Kahlau, Rothbart, Thiele, Lein, Bock, Grimm, Schöttler: "LCAA, a novel factor required for magnesium protoporphyrin monomethylester cyclase accumulation and feedback control of aminolevulinic acid biosynthesis in tobacco." in: Plant physiology, Vol. 160, Issue 4, pp. 1923-39, (2012) (PubMed).

Lundin, Nurmi, Rojas-Stuetz, Aro, Adamska, Spetea: "Towards understanding the functional difference between the two PsbO isoforms in Arabidopsis thaliana--insights from phenotypic analyses of psbo knockout mutants." in: Photosynthesis research, Vol. 98, Issue 1-3, pp. 405-14, (2008) (PubMed).

Antigendetails

Target: PsbO

Hintergrund: AGI Code: At5g66570
The PsbO protein is an extrinisic subunit of the water splitting photosystem II (PSII) complex. The protein is exposed on the luminal side of the thylakoid membrane, and is hihgly conserved in all known oxygenic photosynthetic organisms. Alternative names of PsbO1 include 33 kDa subunit of oxygen evolving system of photosystem II, OEC 33 kDa subunit, 33 kDa thylakoid membrane protein, manganese-stabilizing protein 1 and for PsbO2 33 kDa subunit of oxygen evolving system of photosystem II, OEC 33 kDa subunit, 33 kDa thylakoid membrane protein, manganese-stabilizing protein 2.

Molekulargewicht: 33 kDa

UniProt: Q9S841, P23321