Western Blotting (WB), Immunohistochemistry (IHC), ELISA
Aufreinigung
S100A9 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
S100A9 antibody was raised against a 15 amino acid synthetic peptide near the internal region of S100A9.
ELISA: Antibody detection limit dilution 1:8000. Western Blot: Approximately 16 kDa band observed in human peripheral blood mononucleocytes lysates (calculated MW of 13.2 kDa according to NP_002956.1). The observed molecular weight corresponds to earlier findings (~15 kDa ) in literature with different antibodies (McCormick et al, Biol Chem. 2005 Dec 16,280 (50) :41521-9., PMID: 16216873). Recommended concentration: 0.3-1 μg/mL. Immunohistochemistry: In paraffin embedded human lung shows nuclear staining in select cells, Recommended concentration, 2-4 μg/mL.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.