This antibody is expected to recognize all three human isoforms of this protein.
Aufreinigung
Nup50 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Nup50 antibody was raised against a 12 amino acid synthetic peptide near the C-Terminus of Nup50.
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 60 kDa band observed in Jurkat lysate (calculated MW 50.1 kDa according to NP_009103.2). Recommended for use at 0.2-1 μg/mL. Immunohistochemistry: In paraffin embedded human uterus shows textured nuclear staining of endometrial cells. Recommended concentration, 2.5-3.8 μg/mL.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.