Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Aufreinigung
MDA5 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
MDA5 antibody was raised against a 13 amino acid synthetic peptide near the N-Terminus of MDA5.
ELISA: Antibody detection limit dilution 1:32000. Western Blot: No signal obtained yet but low background observed in human placenta lysate at up to 1 μg/mL. Immunohistochemistry: In paraffin embedded human pancreas, shows strong cytoplasmic staining of some cells in the islets of Langerhans. Recommended concentration, 10 μg/mL.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Minimize freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Aliquot and store at -20 °C.
Target
IFIH1
(Interferon Induced with Helicase C Domain 1 (IFIH1))